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云南滇南小耳猪与广西巴马小型猪mtDNA D-Loop的初步研究
引用本文:倪丽菊,谢建云,胡建华.云南滇南小耳猪与广西巴马小型猪mtDNA D-Loop的初步研究[J].实验动物与比较医学,2011,31(3):207-210.
作者姓名:倪丽菊  谢建云  胡建华
作者单位:上海市实验动物质量监督检验站;上海实验动物研究中心,上海201203
基金项目:上海市科技发展基金资助项目
摘    要:目的研究封闭群滇南小耳猪与广西巴马小型猪线粒体DNA(mtDNA)的多态性,探索小型猪遗传监测的方法。方法用18种限制性内切酶对23头滇南小耳猪和21头广西巴马小型猪mtDNA D-loop的PCR产物进行RFLP分析。结果滇南小耳猪和广西巴马小型猪的mtDNA D—loop在品种内和品种间均表现出相同的酶切图谱,未见多态性。结论滇南小耳猪与广西巴马小型猪mtDNA D—loop的遗传多样性贫乏,mtDNA PCR—RFLP技术在其遗传监测中不具应用价值。

关 键 词:滇南小耳猪  广西巴马小型猪  mtDNA  D—loop  多态性  PCR-RFLP

Preliminary Study on mtDNA D-loop of Yunnan Diannan Small-ear Pigs and Guangxi Bama Miniature Pigs
NI Li-ju,XIE Jian-yun,HU Jian-hua.Preliminary Study on mtDNA D-loop of Yunnan Diannan Small-ear Pigs and Guangxi Bama Miniature Pigs[J].Laboratory Animal and Comparative Medicine,2011,31(3):207-210.
Authors:NI Li-ju  XIE Jian-yun  HU Jian-hua
Institution:1. Shanghai Quality Monitoring Centre for Laboratory Animals; 2. Shanghai Laboratory Animal Research Center, Shanghai 201203, China)
Abstract:Objective To research the polymorphism of mtDNA in the outbred Diannan small-ear pigs and Guangxi Bama miniature pigs, and to discuss the methods of genetic monitoring on the miniature pigs. Methods The mtDNA D-loop of Diannan small-ear pigs and Guangxi Bama miniature pigs were amplified and analyzed using 18 restriction edonucleases by RFLP. Results The restriction cleavage patterns of the mtDNA D-loop showed monomorphism among or within the breeds of Diannan small-ear pigs and Guangxi Bama miniature pigs. Conclusion The genetic diversity of Diannan small-ear pigs and Guangxi Bama miniature pigs was poor, and the mtDNA PCR-RFLP techniques couldn't be applied to genetic monitoring of them.
Keywords:mtDNA D-loop  PCR-RFLP
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