以功能磁共振成像为主综合评价大鼠局灶性脑缺血模型究

目的采用功能核磁为主综合评价大鼠局灶性脑缺血模型，以期寻找一种准确评价大鼠局灶性脑缺血（MCAO）模型的方法，为后续实验提供一致性较好的模型。方法采用功能磁共振成像（fMRI）检测MCAO模型的梗死部位、血管阻塞、神经纤维束等情况，对照Bederson评分、神经症状的综合行为学评分及脑TTC染色综合判断MCAO模型。结果模型组大鼠术后fMRI检测显示，术侧颞顶叶及外侧出现明显水肿带，CCA、ICA、ECA完全堵塞，神经纤维束稀少，部分纤维束中断、缺失。等级相关性分析显示，神经功能缺失评分与脑梗死比例具有相关性（rs=0．879，P〈0．01）。模型组前肢屈曲、躯体屈曲、前肢翻转、后肢翻转、自发活动能力、竖板攀爬、平衡功能等各项神经症状的综合行为学评分与假手术组均有显著差异（P〈0．01）。结论采用fMRI检测结果，同时结合常规神经症状评分以及TTC染色切片，综合分析其结果，可准确判断活体状态下大鼠脑卒中模型的损伤部位和程度，筛选出一致性较好的大鼠脑卒中模型。

Study on Comprehensive Evaluation of Rats' MACO Model Mainly by fMRI

Objective To search an exact method to evaluate the middle cerebral artery occlusion （MACO） model and provide a more consistent model for followed experiments, the functional magnetic resonance was mainly applied to comprehensively evaluate the rat＇s focal cerebral ischemia model. Method The MACO model was synthetically judged by Bederson score comparation, the comprehen- sive behavioral score of neural symptoms, the brain TTC staining and mainly by the functional magnetic resonance imaging （fMRI） which was applied to detect the infract position, angiemphraxis and nerve fiber bundle. Result The fMRI detection on the model group rats was showed that the significant edema was appeared in surgical and lateral sides of temporal parietal lobe, meantime, CCA, ICA, ECA were completely blocked and nerve fibers bundles were rare, even some of them were broken off or missed. Rank correlation analysis was showed that the nervous deficit scores and cerebral infarction were in the proportion of relevant （rs=0.879, P〈0.01）. Each nervous symptom in the model group has significant differences compared with the sham operation group （P〈0.01）. Conclusion The fMRI, combined with the standard nervous symptom score and TIC staining can accurately determine the injury location and extent of damage in stroke model rats in vivo, so that the consistent stroke model rats can screen out finally.