巴马香猪Toll样受体4基因cDNA的克隆及生物信息学分析

目的观察三聚氰胺急性毒性及中药三金汤对三聚氰胺的清除作用。方法昆明小鼠90只，分为7组灌胃三聚氰胺，2组对照，观察其对小鼠的毒性。昆明小鼠70只分为7组，三金汤预防高、中、低剂量组，三金汤治疗组、三聚氰胺模型组、2组对照，一次性给予三聚氰胺后，观察三金汤对小鼠肾和肺三聚氰胺的清除作用。结果昆明小鼠灌胃三聚氰胺的LD50为9366mg／kg，肾脏未见结晶，肾、肝、肺有炎性细胞浸润。三聚氰胺灌胃后在肾、肺含量分别为327ng／g、177．5ng／g，三金汤预防高剂量组清除三聚氰胺效果最佳，肾、肺三聚氰胺含量分别降低至58ng／g、28．2ng／g。结论本实验条件下，初步认定三聚氰胺属微毒或无毒；中药三金汤对小鼠体内三聚氰胺的清除作用以三金汤预防高剂量组的效果最佳。

Cloning and Bioinformatics Analysis of TLR4 cDNA in Bama Miniature Pig

Objective To observe the acute toxicity of melamine and the detoxification of San Jin Tang in Kunming （KM） mice. Methods Ninety SPF KM mice （half male and female） were divided into seven groups administered with different dosage of melamine and two blank control groups. The toxic reaction to the mice caused by melamine was observed, and 70 SPF KM mice were divided into high, middle and low dosage of San Jin Tang（三金汤） administered preventive groups,San Jin Tang treatment group, melamine model group and two control groups. The detoxification of San Jin Tang in kindey and lungs after of mice administered with melamine one time was observed. Results The LD50 of melamine to KM mice was 9366 mg/kg. There were no crystallization in kidney but inflammatory cells infiltration appeared in kidney, liver and lung.The content of melamine in kidneys and lung was 327ng/g and 177.5 ng/g respectively. The high dosage of San Jin Tang had best effects on cleaning up the melamine in kidney and lung, reduced to 58 ng/g and 28.2 ng/g, respectively. Conclusion The preliminary experiments suggested that the melamine was micro-toxic or non-toxic. The higher dose group of Chinese Medicine San Jin Tang showed best effect to get rid of melamine in mice.