| 2型糖尿病大鼠的尿液代谢组学改变 |
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| 引用本文： | 袁琳,白永生,周明眉,胡娜,陆雄.2型糖尿病大鼠的尿液代谢组学改变[J].实验动物与比较医学,2011,19(2). |
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| 作者姓名： | 袁琳 白永生 周明眉 胡娜 陆雄 |
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| 作者单位： | 上海中医药大学科技实验中心,上海,201203 |
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| 基金项目： | 上海市重点学科建设项目，上海高校选拔培养优秀青年教师科研专项基金 |
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| 摘 要： | 目的 通过检测2型糖尿病大鼠尿液代谢谱的变化.探讨代谢组学在糖尿病研究中的应用.方法 SD大鼠高糖高脂饲料喂养6周后,腹腔注射链脲菌素(Streptozotocin,STZ)37 mg/kg建立2型糖尿病模型,动态检测空腹血糖(FBG)变化,检测甘油三酯(TC)、总胆固醇(TC)、游离脂肪酸(FFA)及胰岛素(INS)水平.分别于造模前、造模后1、2、4、6、8周收集大鼠尿液,采用氯甲酸乙酯(ECF)衍生和气相色谱质谱联用(GC/MS)的方法检测大鼠尿液代谢谱的变化.结果 造模后大鼠FBG持续升高,FFA、TG、TC明显升高,血清INS含量明显降低;造模后,模型组大鼠代谢谱与对照组完全区分,并随时同变化逐渐偏离;比较两组差异,从差异变量中鉴定出10个生物学标记物.结论 高糖高脂喂养联合小剂量STZ造模2型糖尿病大鼠尿液代谢组学发生明显变化,尿液代谢组学在一定程度上反映2型糖尿病大鼠的病理变化.
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| 关 键 词： | 2型糖尿病 大鼠 高糖高脂喂养 链脲菌素 代谢组学 |
Urinary metabonomic variation in type 2 diabetic rats |
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| YUAN Lin,BAI Yong-sheng,ZHOU Ming-mei,HU N,LU Xiong.Urinary metabonomic variation in type 2 diabetic rats[J].Laboratory Animal and Comparative Medicine,2011,19(2). |
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| Authors: | YUAN Lin BAI Yong-sheng ZHOU Ming-mei HU N LU Xiong |
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| Institution: | YUAN Lin,BAI Yong-sheng,ZHOU Ming-mei,HU Na,LU Xiong(Experiment Center of Science and Technology,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China) |
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| Abstract: | Objective To investigate the urinary metabonomic variation in diabetic rats in order to explore the ap-plication of metabonomics in research of diabetes.Methods Male Sprague-Dawley rats were fed with high-fat and high-su-crose diet for 6 weeks and then injected with vehicle or streptozotocin(STZ) in a dose of 37 mg /kg.Fasting blood glucose(FBG) was monitored weekly after STZ injection.Urines of normal rats and model rats were collected at 1,2,4,6,8 weeks after STZ injection.Serum insulin(INS),triglyceride(... |
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| Keywords: | Type 2 diabetes Rat high-fat High-sucrose diet Streptozotocin Metabonomics |
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