改进原位接种技术建立裸小鼠人大肠癌（SW1116）模型

为改进裸小鼠肠原位肿瘤接种方法和提高肿瘤转移发生，用自上肿瘤肠避粘接和脾切除技术，在裸小鼠体内筛选并建立稳定的，有转移能力的模型要现，对裸小以下接种后生长的无转移移能力的人ＳＷ１１１６大肠癌组织块进行大部切除，可诱发肿瘤转移，用肿瘤肠壁原位粘接技术可获得肿瘤的自发转移个别，同时合作脾切除技术更可获得肿瘤的高转移。结果提示，人大肠癌的转移可在裸小本仙诱导增强肿瘤肠壁原位 妆及脾切除是研究建立人大肠主

A New Orthotopic Transplantation Technique for Establishing Human Colon Cancer SW1116 Model in Nude Mice

To establish a human colon cancer model in nude mice, a new anastomotic orthotopic transplatation technique was developed. Human colon cancer SW1116 cells were implanted subcutaneosly and passaged in nude mice for 4 generations ,no metastasis was found. From 5th generation the tumors were resected subtotally when reaching 1.5 cm in diameter. After the tumor grew again,1 of the 8 mice appeared metastatic lymph node in the armpit of the forelimb. A medical adhesive, OB Glue, was used in orthotopic transplantation of the intact SW1116 tumors from the metastatic lymph node. At the first 4 passages the metastatic rate was low. From the 5th passage, the spleen of mice were also resected at the time of tumor orthotopic transplantation, with which the metastatic rate was high. From 6th to 10th passage the metastatic rate was 100%(40/40) to the mesenteric lymph nodes, 75%(30/40) to the livers. It can be considered that the metastatic SW1116 sub population (subpop) was obtained. The orthotopic Transplantation method and SW1116 subpop model of nude mice may be useful for further study of human colon cancer.