润肠通便功能试验中造模剂的选择

目的 以地芬诺酯为参照,研究洛哌丁胺作为润肠通便功能实验造模剂的有效性、合理性.方法 筛选地芬诺酯与洛哌丁胺在润肠通便功能实验中作为造模剂的适宜剂量.同时用市售具有润肠通便功能的保健食品进行动物实验验证.均进行小肠运动实验和排便实验,并进行统计学分析.结果 （1）筛选试验：在筛选剂量下,两种造模剂的小肠墨汁推进率均小于正常对照组（P＜0.05）,即认为该两种造模剂均可成功建立小肠蠕动抑制模型;在筛选剂量下,洛哌丁胺（9 mg/kg和10 mg/kg）的便秘模型均造模成功,而地芬诺酯便秘模型造模均不成功.（2）验证试验：分别用相同剂量的地芬诺酯、洛哌丁胺剂造模,模型均成立,且受试物的小肠墨汁推进率均大于各自的模型对照组（P＜0.01）,受试物的首便时间、6h粪便粒数和6h粪便重量均明显多于各自模型对照组（P＜0.01）;认为两种造模剂在评价该受试物具有润肠通便功能的作用和效果一致.结论 洛哌丁胺在适宜剂量下可作为润肠通便功能评价方法的造模剂.

Selection of Modeling Drugs for Assessment of Facilitating Feces Excretion Function

Objective To evaluate the validity and rationality of loperamide as the modeling agent to asses the effect of relaxing bowel using diphenoxylate as a reference.Methods The appropriate dosages of diphenoxylate and loperamide as modeling agents were screened.The validation tests were performed with the same health food which had been sold as having the facilitating feces excretion function.The small intestine movement experiment and the defecation experiment were carried out.Results （1） Screening tests：Within the screening doses,the ink propulsion rate of the two modeling drugs were less than the control group （P＜0.05）,so both of the small intestine peristalsis inhibition were established successfully.Within the screening doses,loperamide at the dose of 9 mg/kg and 10 mg/kg induced the constipation,none of diphenoxylate.（2） Validation tests：The animal models were succeeded with the same dosage of diphenoxylate and loperamide.The ink propulsion rates of the test substance were greater than their model control groups respectively （P＜0.01）.The first defecating time,the amount of feces and fecal weight within 6h of the test substance were significantly different with their model control groups （P＜0.01）.Loperamide and diphenoxylate have the same effect of functional food.Conclusion Loperamide can be used as amodeling drug for assessing the facilitating feces excretion function.in the appropriate dose.