犬MC1R基因R306ter与毛色性状相关性研究

目的　分析犬MC1R基因中R30 6ter位点多态性与犬毛色表型的相关性 ,为遗传育种 ,培育出更加优良的实验用犬奠定基础。方法　采用PCR SSCP技术 ,对MC1R基因R30 6ter位点进行基因多态性检测 ,分析位点多态性与毛色性状之间的相关性 ,并对该位点进行克隆测序。结果　PCR SSCP分析结果表明 ,R30 6ter位点序列具有多态性 ,表现为C、D二个等位基因和CC、CD及DD三种基因型。对R30 6ter多态性片段克隆测序发现 ,MC1R基因在编码第 30 6位氨基酸的密码子处存在一个由CGA到TGA的终止突变。结论　经统计分析结果表明在杂种犬中MC1R基因多态性与毛色性状不存在显著的相关性 ,这可能是由于外科手术学教学用犬是杂种犬 ,其遗传背景不同所致。由于MC1R基因的R30 6ter位点内存在碱基变异 ,因此在杂种犬中表现出明显的PCR SSCP多态性

Study on the Relationship between R306ter Loci in MC1R Gene and Coat Color Trait in Dogs

Objective The relationship between the genetics polymorphism and phenotype of dog coat color was analyzed in order to give advice for the dog breeding. Methods The loci of R306ter in the canine MC1R gene were detected through the technology of PCR SSCP, and the relationships between the polymorphism of R306ter and coat color trait were analyzed. Furthermore, fragments with the polymorphism at R306ter were sequenced. Results\ By the method of PCR SSCP, the R306ter polymorphism was found with two alleles C and D and three genotypes CC, CD and DD. According to the results of sequencing of R306Ter, one terminal change from CGA to TGA at the position 306 was found. Conclusion\ According to the statistical analysis, no significant associations between the polymorphism of MC1R gene and the coat color were found and these results may be due to the differences of genetic background. For the variations of R306ter loci in MC1R gene, the polymorphism of PCR SSCP in the cross breed canine was detected.