多重PCR在几个近交系小鼠遗传检测中的应用初探

目的　探讨多重PCR方法在小鼠微卫星检测中的应用。方法　用 34对特异性引物对AKR、BALB c、C57 BL、DBA 2、CBA、A WY、6 15、T739、BALB cJ和AKR J 10个品系的近交系小鼠用PCR方法进行遗传检测 ,并从中选用 4对引物 ,对这些品系小鼠进行二重和多重PCR检测。结果　二重PCR在与单一PCR相同的反应条件下 ,扩增出两条与预期条带相同的带 ,而三重PCR则没有得到三条预期的条带 ,出现了干扰现象。结论　通过二条条带的距离可以鉴别出不同的近交系小鼠 ,二重PCR可应用于近交系小鼠的遗传检测 ,具有方便简单、省时的优点

Genetic Monitoring in Ten Inbred Strains of Mice by Multiplex PCR

Objective To explore the application of multiplex PCR method in the detection of microsatellite in mice. Methods\ Four pairs of specific primers were selected to perform multiplex DNA amplification in ten inbred stains of mice. Results\ Duplex DNA amplification got two expected bands under the same condition of the PCR reaction, but triplex DNA amplification did not get three expected bands.Conclusion\ The duplex DNA amplification is a fast and economical method to analyse genotype of inbred mice.