靶向4-1BBL基因siRNA抑制HL-60B细胞生长的体外研究

目的：观察4-1BBL基因小分子干扰RNA（siRNA）对HL-60细胞生长及诱导其凋亡作用的影响。方法：化学合成法合成靶向人4-1BBL基因siRNA,脂质体转染法转染HL-60B细胞;半定量PCR和流式细胞术检测转染前后HL-60B细胞4-1BBL的表达水平,MTT法检测HL-60B细胞增殖,流式细胞术检测细胞凋亡。结果：靶向4-1BBL基因siRNA转染HL-60B细胞后,4-1BBLmRNA和蛋白的表达明显降低;细胞增殖被抑制,其抑制作用在转染后48h最明显,增生抑制率达到（22.1±2.3）%;细胞凋亡率增加,由（9.8±2.5）%上升到（32.5±5.1）%。结论：化学合成的siRNA在体外能成功抑制靶基因4-1BBL的表达和HL-60B细胞的增殖,并有助于细胞趋向凋亡。

4-1BBL small interfering RNA inhibits the proliferation of HL-60B cell in vitro

Objective：To observe the influence of 4-1BBL small interfering RNA（siRNA） on the growth and apoptosis of HL-60B cells.Methods：The siRNA for 4-1BBL gene was designed and synthesized in vitro,and then transfected into HL-60B cells by Lipofectamine;the expression of 4-1BBL mRNA and protein was detected by RT-PCR and flow cytometry,respectively;the proliferative rate of HL-60B cells was determined by methyl thiazoy tetralium（MTT）,and the cell apoptosis was analyzed by flow cytometry.Results：After the siRNA for 4-1BBL gene was transfected into HL-60B cells,the expression of 4-1BBL gene and protein decreased,the growth of cells was repressed and the proliferation rate of HL-60B cells decreased.The inhibition rate was（22.1±2.3） % 48 h after transfection;the apoptosis rate increased from（9.8±2.5） % to（32.5±5.1） %.Conclusions：Chemically synthetic siRNA can decrease 4-1BBL gene expression,inhibit cellular proliferation and promote apoptosis in vitro.