| 大鼠骨髓内皮祖细胞移植到裸鼠体内的分化 |
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| 引用本文： | 张社红,项平.大鼠骨髓内皮祖细胞移植到裸鼠体内的分化[J].蚌埠医学院学报,2008,33(6):637-639. |
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| 作者姓名： | 张社红 项平 |
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| 作者单位： | 蚌埠医学院第一附属医院,中心实验室,安徽,蚌埠,233004;蚌埠医学院第一附属医院,中心实验室,安徽,蚌埠,233004 |
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| 基金项目： | 安徽省教育厅自然科学基金
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| 摘 要： | 目的：研究骨髓内皮祖细胞（EPCs）在裸鼠体内分化情况,进一步揭示其生物学特性。方法：密度梯度离心法分离大鼠骨髓EPCs;Hoechst33342标记原代（第10天）的EPCs注射到裸鼠背部两侧皮下,观测注射部位的变化及生成物的大小;移植第50天,剥离EPCs形成的皮下肿物,HE及免疫荧光染色,检测细胞CD31、Flk1、CD34的表达情况。结果：裸鼠皮下接种EPCs第20天,形成（0.13±0.018）cm^2球形皮下肿物,逐渐增大,第40天开始处于生长平台期;HE染色组织内有管腔样结构、肌肉样组织及脂肪样细胞,同视野荧光显微镜下可见Hoechst标记的细胞;免疫荧光检测显示,Hoechst标记细胞抗原CD31、Flk1、CD34表达阳性。结论：大鼠骨髓EPCs移植到裸鼠体内一部分分化为内皮样细胞,并形成管腔样结构,另一部分分化为非内皮系细胞。
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| 关 键 词： | 新生血管 干细胞 内皮祖细胞 分化 细胞移植 裸鼠 大鼠 |
Differentiation of rat marrow-derived endothelial progenitor cells transplanted into nude mice |
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| ZHANG She-hong,XIANG Ping.Differentiation of rat marrow-derived endothelial progenitor cells transplanted into nude mice[J].Journal of Bengbu Medical College,2008,33(6):637-639. |
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| Authors: | ZHANG She-hong XIANG Ping |
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| Institution: | ZHANG She-hong,XIANG Ping(Central Laboratory,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China) |
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| Abstract: | Objective:To study on differentiation of marrow-derived endothelial progenitor cells(EPCs)in vivo,and to further reveal EPCs' biological characteristics.Methods:Rat bone marrow-derived EPCs were separated by density gradient centrifugation,and cultrued in vitro;and then labelled with Hoechst 33342 and collected.The labelled EPCs were injected subcutaneously into nude mice's back,on the both sides of the spinal.Animals were observed daily and measured the size of the subcutaneous masses.At the 50th day after... |
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| Keywords: | neovascula tization stem cell endothelial progenitor cells differentiation cell transplantation mice nude rats |
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