Ezrin蛋白与E-cadherin在食管鳞癌中的联合检测及其意义

目的：探讨食管鳞状细胞癌（ESCC）组织中Ezrin蛋白与E-cadherin的表达及其与肿瘤侵袭和转移的关系.方法：以30例癌旁正常黏膜组织作对照,应用免疫组织化学SP法检测76例ESCC组织标本中Ezrin蛋白与E-cadherin的表达.结果：ESCC组织中Ezrin蛋白与E-cadherin的异常表达率分别为78.95％和59.21％;而癌旁正常黏膜组织中异常表达率分别为26.67％和6.67％,两项指标差异均有统计学意义（P＜0.01）.Ezrin蛋白异常表达在ESCC淋巴结转移和组织分化程度间差异有统计学意义（P ＜0.05和P＜0.01）,E-cadherin异常表达在ESCC组织的浸润深度、ESCC细胞的分化程度和淋巴结转移间差异均有统计学意义（P ＜0.05 ～P ＜0.01）.Ezrin蛋白与E-cadherin在ESCC中的异常表达呈正相关关系（P＜0.05）.E-cadherin异常表达组Ezrin蛋白表达阳性率显著高于E-cadherin正常表达患者（P＜0.05）.两者同时异常表达的40例中淋巴结转移高达70％,而两者同时正常表达的11例均无淋巴结转移,差异有统计学意义（P＜0.01）.结论：Ezrin蛋白与E-cadherin在ESCC组织中均异常表达,且呈正相关.联合检测Ezrin蛋白和E-cadherin的表达有助于综合判断ESCC的浸润、转移及预后.

The combining detection of Ezrin protein and E-cadherin in esophageal squamous cell carcinoma and its significance

Objective ：To investigate the expressions of Ezrin protein and E-cadherin in esophageal squamous cell carcinoma（ESCC） and their correlations with the invasion and metastasis of tumor. Methods ： The expressions of Ezrin protein and E-cad in 76 ESCC tissue specimens and 30 normal tissue specimens were detected by SP method. Results： The abnormal expression rates of Ezrin protein and E- cadherin in ESCC tissue and normal tissue were 78.95% ＆ 59.21% and 26.67% ＆ 6.67%, respectively, the differences of two indicators were statistical significance（ P 〈 0.01 ）. The differences between the abnormal expression of Ezrin protein and the lymph node metastasis and tissue differentiation degree of esophageal squamous carcinoma were statistical significance （P 〈 0.05 and P 〈 0.01 ）, the differences between the abnormal expression of E-cadherin and the invasion depth, lymph node metastasis and tissue differentiation degree of esophageal squamous carcinoma were statistical significance（ P 〈 0.05 to P 〈 0.01 ）. There was a positive correlation between the abnormal expression of Ezrin protein and E-cadherin in ESCC （ P 〈 0.05 ）. The positive expression rate of Ezrin protein in tissue specimens with abnormal E-cadherin expression was significantly higher than that in tissue specimens with normal E-cadherin expression （P 〈 0.05）. The lymph node metastasis rate in 40 tissue specimens with abnormal E-cadherin and Ezrin protein expressions was 70% , no lymph node metastasis in 11 tissue specimens with normal E-cadherin and Ezrin protein expressions was found, the difference of which was statistical significance （P 〈 0.01 ）. Conclusions： The abnormal expressions of Ezrin protein and E-cadherin in ESCC are positively correlated relationship. The combining detection of Ezrin protein and E-cadherin can help to judge the metastasis and prognosis of ESCC.