舒林酸对胃癌细胞株BGC-823作用的实验研究

目的:研究舒林酸对胃癌细胞株BGC一823增殖、凋亡的作用,探讨其作用机制. 方法:体外培养的胃癌细胞株BGC-823加入不同浓度的舒林酸作用不同时间后,用倒置显微镜观察细胞形态学变化,四甲基噻唑氮蓝(MTT)法检测细胞生长衣抑制率，流式细胞术(flow cytometry,FCM)检测细胞周期;透射电镜观察细胞超微结构变化及凋亡形态变化,DNA电泳检测细胞凋亡DNA片段. 结果:舒林酸可以改变胃癌细胞株BGC-823的形态,抑制其生长,影响其细胞周期分布,透射电镜观察到细胞凋亡的形态,DNA电泳检测出典型的细胞凋亡的特征性梯状条带. 结论:舒林酸有抑制胃癌细胞株BGC-823增殖,诱导其凋亡的作用,并且可能与舒林酸影响其细胞周期分布、抑制了环氧合酶-2(COX-2)表达有关.

Experimental study for effect of sulindac for gastric cancer cell BGC-823

Objective:To study the effects of sulindac on proliferation and apoptosis in gastric cancer cells and the probable mechanism involved.Methods:The BGC-823 cells were cultured with sulindac at various concentrations for different time.Morphological changes of BGC-823 cells were observed by inversion microscopy.The inhibitory rate of cell growth was assessed by MTT spectrophotometric analysis.The cell cycle distribution was measured by flow cytometry(FCM).The ultrastructure of cells and the apoptotic morphology were examined by transmission electronic microscopy.Apoptosis of the cells was examined by DNA electrophoresis.Results:Sulindac can make gastric cancer cells BGC-823 morphologic change,inhibit its growth and change the cells cycle distribution.The apoptotic morphology of BGC-823 cells were observed by transmission electronic microscopy.The notably DNA ladders of the cells apoptosis were detected by DNA electrophoresis.Conclusions:Sulindac can inhibit the BGC-823 cell proliferation and induce cell apoptosis,the mechanism may be related to changing cell cycle distribution and downregulate the expression of COX-2.