结肠癌组织中p53凋亡刺激蛋白家族表达水平及其与临床病理学特征的相关性研究

目的:探讨结肠癌组织中p53凋亡刺激蛋白(ASPP)家族表达水平及其与临床病理学特征的相关性.方法:选取结肠癌患者的结肠癌组织共50例,以及健康人的正常结肠组织共30例.分析ASPP家族与结肠癌的组织分化程度、浸润深度和淋巴结转移等临床特征的相关性.结果:结肠癌组织与正常结肠组织中ASPP1和ASPP2的阳性表达率差异均无统计学意义(P>0.05);而结肠癌组织中iASPP的阳性表达率显著高于正常结肠组织(P<0.05);结肠癌组织中iASPP的阳性表达率在肿瘤病理分级间差异均无统计学意义(P>0.05),而ASPP1和ASPP2的阳性表达在肿瘤病理分级间差异均有统计学意义(P<0.05和P<0.01);结肠癌组织中ASPP1与iASPP的阳性表达在肿瘤浸润深度间差异均无统计学意义(P>0.05),ASPP2的阳性表达在肿瘤浸润深度间差异有统计学意义(P<0.05);而结肠癌组织中ASPP1、ASPP2和iASPP的阳性表达均在淋巴结有无转移间差异均无统计学意义(P>0.05).结论:iASPP在结肠癌组织和正常组织的阳性表达的差异,表明其有可能成为结肠疾病良、恶性的诊断及鉴别指标之一;ASPP2的阳性表达与结肠癌病理分期和分级有相关关系,表明其有可能成为结肠癌预后指标之一.

The expression of ASPP family genes and its correlation with clinical pathological features in colon cancer tissue

Objective:To explore the expression levels of apoptosis stimulating protein of P53(ASPP) family genes in colon cancer tissue,and its correlation with clinical pathological features. Methods:Fifty colon cancer tissue specimens and 30 normal colon tissue specimens were investigated. The correlations of the ASPP family with the differentiation,depth of invasion and lymph node metastasis of colon cancer were analyzed. Results:The differences of the expressions of ASPP1 and ASPP2 between the colon cancer and normal colon tissue were not statistically significant(P>0. 05),and the positive expression rate of iASPP in colon cancer was significantly higher than that in normal colon tissue(P <0. 05). The differences of the positive expression rate of iASPP in different tumor pathological grading was not statistically significant(P>0. 05),and the differences of the positive expression rate of ASPP1 and ASPP2 in different tumor pathological grading were statistically significant(P<0. 05 and P<0. 01). The differences of the positive expression rates of ASPP1 and iASPP in different tumor invasion depth were not statistically significant(P >0. 05),and the difference of the positive expression rate of ASPP2 in different tumor invasion depth was statistically significant(P <0. 05). The differences of the positive expression rates of ASPP1,ASPP2and iASPP in different lymph node metastasis of tumor were not statistically significant(P>0. 05). Conclusions:The positive expression of iASPP between colon cancer and normal tissue is different,which may become one index in the diagnosis and differential diagnosis of colon malignant and benign diseases. The positive expression of ASPP2 is correlated with the pathological stage and grade of colon cancer,which may be one prognostic indicator of colon cancer.