胰岛素样生长因子-1对成骨细胞增殖影响的实验研究

目的：研究不同浓度胰岛素样生长因子－1（IGF－1）对兔成骨细胞增殖的影响。方法：采用组织块细胞培养技术进行兔成骨细胞分离培养，取第2代成骨细胞分别与0．1ng/ml,1ng/ml,10ng/ml,20ng/mlIGF-1培养，培养24、36小时后行四唑盐比色法检测细胞增殖情况。结果：IGF－1与成骨细胞培养24、36小时，经MTT法检测，不同浓度IGF－1与对照组比较，有显著性差异（P＜0．01）。IGF－1浓度为0．1、1、10ng/ml，各组之间相比存在显著性差异（P＜0．05），结论：IGF－1对成骨细胞有明显促进增殖作用，在0．1－10ng/ml范围，存在浓度的依赖性。

Effects of Insulin-like Growth Factor-1 on Proliferation of Rabbit Osteoblast in Vitro.

Objective To investigate and compare the effect of insulin-like growth factor-1 on proliferation of rabbit osteoblast in vitro. Methods Osteoblasts were obtained from rabbit costal bone by explant culture technology. The second subculture was exposed to insulin-like growth factor-1 at variant concentrations of 0,0.1,1,10and 20ng/ml for 24 and 36 hours and MTT assay was employed to observe the effects of insulin-like growth factor-1 on osteoblast proliferation. Results Osteoblasts exposed to insulin-like growth factor-l at variant concentrations of 0.1,1,10,20ng/ml were significantly different compared with the control ( P <0.01),and significant differences were found between every two groups when osteoblasts were exposed to insulin-like growth factor-l at range from 0.1 to 10ng/ml ( P <0.05).Conclusions Insulin-like growth factor-l could significantly elevate the proliferation of osteoblasts. At range from 0.1-10ng/ml, It depended on concentration. MTT assay is a good kind of method for determining the proliferation of osteoblasts.