| 14-3-3基因真核表达载体的构建 |
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| 引用本文： | 苗新东,刘同美,王婷,陈安琪,崔晓栋,郭军堂.14-3-3基因真核表达载体的构建[J].湖北民族学院学报(医学版 ),2009,26(4):10-13. |
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| 作者姓名： | 苗新东 刘同美 王婷 陈安琪 崔晓栋 郭军堂 |
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| 作者单位： | 潍坊医学院病理生理学教研室,山东,潍坊,261053 |
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| 摘 要： | 目的克隆人14-3-3基因并构建真核表达载体pcDNA3.1（＋）-14-3-3。方法从胎脑组织中提取总RNA,用RT-PCR方法获得人14-3-3基因的全长cDNA,将其插入pCUm-T载体中;序列测定后,重组携带14-3-3基因的表达载体pcDNA3.1（＋）-14-3-3。结果经限制性内切酶酶切分析、PCR和DNA序列测定证实目的基因已插入重组质粒。结论成功构建了14-3-3真核表达载体pcDNA3.1（＋）-14-3-3,为进一步研究14-3-3在帕金森病中的作用奠定了良好的基础。
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| 关 键 词： | 14-3-3基因 真核表达 帕金森病 |
Construction of the Eukaryotic Expression Vector of 14-3-3 |
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| MIAO Xin-dong,LIU Tong-mei,WANG Ting,et al..Construction of the Eukaryotic Expression Vector of 14-3-3[J].Journal of Hubei Institute For Nationalities(Medical Edition),2009,26(4):10-13. |
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| Authors: | MIAO Xin-dong LIU Tong-mei WANG Ting |
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| Institution: | MIAO Xin-dong,LIU Tong-mei,WANG Ting,et al.(Department of Pathophysiology,Weifang Medical University,Weifang 261053,China) |
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| Abstract: | Objective To clone human 14-3-3 gene and construct the eukaryotic expression vector of pcDNA3.1(+)-14-3-3.Methods Total RNA was isolated from human brain tissue.The full-length human14-3-3 cDNA was obtained by RT-PCR and then inserted into pCUm-T vector for sequencing.The correct gene was subcloned into pcDNA3.1(+) to generate recombinant eukaryotic expression vector pcDNA3.1(+)-14-3-3.Results Enzyme digestion analysis PCR and sequencing showed that the target gene was cloned into recombinant vector.Conclus... |
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| Keywords: | 14-3-3 gene Eukaryotic expression Parkinson's Disease |
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