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芍药苷对放射线致血虚证小鼠骨髓Epo和G-CSF基因表达的影响
引用本文:郭平,王继峰,王升启.芍药苷对放射线致血虚证小鼠骨髓Epo和G-CSF基因表达的影响[J].山东中医药大学学报,2005,29(3):236-239.
作者姓名:郭平  王继峰  王升启
作者单位:1. 北京中医药大学,北京,100029
2. 军事医学科学院,放射医学研究所,北京,100850
基金项目:国家自然科学基金项目 (3 0 2 71617),军队“十五”重点项目 (0 1Z0 19),北京市“二四八”重大创新工程项目(H0 10 2 10 2 2 0 113 )
摘    要:目的:考察芍药苷对放射线致血虚证小鼠的生血作用及对骨髓Epo、G CSF基因表达的影响,为阐明芍药苷补血作用的分子机制提供依据。方法:采用3.5Gy60 Coγ射线全身1次性照射,制备小鼠血虚证模型,检测外周血象,骨髓集落培养观察并计数CFU GM、BFU E、CFU E、CFU mix ,RT PCR观察并检测骨髓Epo和G CSF基因表达。结果:芍药苷显著升高放射线致血虚证小鼠外周血白细胞数量和骨髓CFU GM、BFU E、CFU E、CFU mix数量,上调骨髓Epo和G CSF基因表达。结论:芍药苷具有生血作用,促进骨髓Epo和G CSF基因的表达可能是其生血作用的机制之一。

关 键 词:芍药苷  辐射  血虚证  造血祖细胞  红细胞生成素  粒细胞集落刺激因子  骨髓  逆转录聚合酶链反应
文章编号:1007-659X(2005)03-0236-04
修稿时间:2004年12月30

EFFECTS OF PAEONIFLORIN ON Epo AND G-CSF GENE EXPRESSION IN BONE MARROW OF IRRADIATED BLOOD DEFICIENCY MICE
GUO Ping,WANG Ji-feng,WANG Sheng-qi.EFFECTS OF PAEONIFLORIN ON Epo AND G-CSF GENE EXPRESSION IN BONE MARROW OF IRRADIATED BLOOD DEFICIENCY MICE[J].Journal of Shandong University of Traditional Chinese Medicine,2005,29(3):236-239.
Authors:GUO Ping  WANG Ji-feng  WANG Sheng-qi
Institution:GUO Ping1,WANG Ji-feng1,WANG Sheng-qi2
Abstract:Objective:to observe the blood enriching function of paeoniflorin (PAE) and its effects on erythropoietin (Epo) and granulocyte colony-stimulating factor (G-CSF) gene expressions in bone marrow of blood deficiency mice,and provide the basis for understanding the molecular mechanism of blood enriching function of PAE. Methods: blood deficiency mice model were established by using 3.5 Gy 60 Co γ-ray. Peripheral blood cells analyzed,bone marrow colony culture were observed and CFU-GM,BFU-E,CFU-E and CFU-mix were counted,and then Epo and G-CSF gene expressions in bone marrow were measured with RT-PCR. Results:PAE can significantly raise the number of peripheral blood cells and the number of bone marrow CFU-GM,BFU-E,CFU-E and CFU-mix of blood deficiency mice and enhance Epo and G-CSF gene expressions in bone marrow. Conclusions:PAE has a function of blood enriching. Promoting Epo and G-CSF gene expressions in bone marrow may be one of the function mechanisms.
Keywords:paeoniflorin  irradiation  blood deficiency  hematopoietic progenitor cell  Epo  G-CSF  bone marrow  RT-PCR
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