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山豆根水提物对体外培养人肝癌细胞增殖及代谢的影响
引用本文:肖正明,宋景贵,徐朝晖,田维明,姜世明.山豆根水提物对体外培养人肝癌细胞增殖及代谢的影响[J].山东中医药大学学报,2000,24(1):62-64.
作者姓名:肖正明  宋景贵  徐朝晖  田维明  姜世明
作者单位:1. 山东师范大学生物系,山东济南,250014
2. 山东中医药大学附属医院,山东济南,250011
基金项目:山东省教委资助课题(J97K09)
摘    要:目的:研究中药山豆根(SSCC)提取物对体外培养的人肝癌细胞的抑癌活性。方法:SSCC经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得SSCC提取物。人肝癌SMMC-7721细胞经SSCC提取物处理不同时间后,以MTT法测SSCC提取物对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。结果:1经SSCC提取物处理48h,其A570光吸收降为对照组的62.1%,72h为9.64%;2SSCC提取物处理7d后,人肝癌细胞数明显低于对照组;3经SSCC提取物处理24h后,在更换为正常培养基恢复培养过程中,细胞数呈现缓慢增长;4经SSCC提取物处理24h后其有丝分裂指数为对照组的50.6%。结论:中药SSCC提取物能抑制体外培养的人肝癌细胞的增殖,降低线粒体代谢活性。

关 键 词:山豆根  肝癌  SMMC-7721  MTT  细胞增殖  线粒体
文章编号:1007-659X(2000)01-0062-03
修稿时间:1999-09-08

EFFECTS OF THE SSCC EXTRACTS ON PROLIFERATION AND MITOCHONDRIA METABOLISM OF HUMAN HEPATOMA SMMC-7721 CELLS
XIAO Zheng-ming,SONG Jing-gui,XU Zhao-hui,TIAN Wei-ming,JIANG Shi-ming.EFFECTS OF THE SSCC EXTRACTS ON PROLIFERATION AND MITOCHONDRIA METABOLISM OF HUMAN HEPATOMA SMMC-7721 CELLS[J].Journal of Shandong University of Traditional Chinese Medicine,2000,24(1):62-64.
Authors:XIAO Zheng-ming  SONG Jing-gui  XU Zhao-hui  TIAN Wei-ming  JIANG Shi-ming
Institution:XIAO Zheng ming ;(Biology depart.,Shandong Teachers Univ.Jinan 250014,China);SONG Jing-ging ;(Affiliated Hosp.,Shandong Univ.of TCM,Jinan, 250012,China);XU Zhao hui ,TIAN Wei-ming ,JIANG Shi-ming ;(Biology depart.,Shandong Teachers Univ.Jinan 250014,China)
Abstract:Objective:To dimonstrate the effects of the extracts from Sophora Subprostrata chun et T chan(SSCC) on the proliferation and mitochondria metabolism of human hepatoma SMMC 7721 cells.Methods:After the test of dose dependent experiment,human hepatoma cells were treated with SSCC extracts at dose of 250 μg/ml for 48 h and mitochondria metabolism activity was detected by MTT method.The experiments of effects of SSCC extracts on the proliferation were performed with the treatments of SSCC extracts at dose of 500 μg/ml for 7 d and the proliferation of human hepatoma cells were also examined after treated with SSCC extracts for 24 h and then replaced by cultured in normal media(containing 10% FCS) for 72 h.Result:(1)SSCC extracts could significantly decrease the activity of mitochondria metabolism,and the A570 absorption in the treatment group was only 62.1% of that in control group after 48 h treatment and 9.64% after 72 h treatment.(2)The human hepatoma cell number in the treatment group was much lower than that in control groups during the two groups were treated for seven days.(3)Had been treated for 24 h then cultured in normal media for 72 h,the human hepatoma cells slowly increased in number;(4)After treated for 24 h,the mitosis index in the treatment group was only 50.6% of that in the control group.Conclusion:SSCC extracts could effectively inhibit the proliferation and mitochondria metabolism activty of human hepatoma cells.
Keywords:Hepatoma SMMC-7721  SSCC  proliferaton mitochondria  MTT
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