探讨维生素C在体外对人宫颈癌HeLa细胞的增殖和凋亡的影响

目的探讨维生素C在体外对人宫颈癌HeLa细胞的增殖和凋亡的影响，以促进维生素C在肿瘤治疗上的应用。方法倒置显微镜下观察其形态学改变；MTT法检测维生素C对HeLa细胞增殖的影响；流式细胞仪分析细胞周期和凋亡率；琼脂糖凝胶电泳分析凋亡过程中细胞基因组DNA的变化。结果维生索C以浓度和时间依赖的方式阻滞HeLa细胞于G0／G1期，抑制其生长并诱导凋亡。结论维生素C可抑制HeLa细胞生长，使细胞阻滞于G0／G1期并诱导细胞凋亡．其原因可能是HPVE6蛋白的表达上调而恢复了P53的功能。

Discuss the influence of vitamin C in vitro which weakly perishes to the person cervical cancer HeLa cell multiplication

Objective Discuss the influence of vitamin C in vitro , which weakly perishes to the person cervical cancer HeLa cell multiplication, in the end to promote vitamin C in the tumor treatment application. Methods Under the inverted microscope observes its morphology, change; MTT assay vitamin C to HeLa cell multiplication influence; Flows the type cell meter analysis cell cycle and weakly perishing rate;applying the agarose gelatin electricity swims analyzes weakly perishes in the process the cell gene group DNA change way to the G0/G1 period, suppresses it to . Results Vitamin C hinders the HeLa cell by the density and the time dependence grow and to induce weakly perishes. Conclusion Vitamin C may suppress the HeLa cell to grow, causes the cell to hinder to the G0/G1 period and induces cell weakly perishing. Its reason possibly was HPV E6 protein expression surrenders to restoring the function of P53 gene.