首页 | 本学科首页   官方微博 | 高级检索  
检索        

高压氧对HIBD新生大鼠学习记忆功能及海马CA1区神经元的远期影响
引用本文:刘小红,赵永利,马巧梅,周熙惠,王燕.高压氧对HIBD新生大鼠学习记忆功能及海马CA1区神经元的远期影响[J].四川大学学报(医学版),2007,38(1):73-77.
作者姓名:刘小红  赵永利  马巧梅  周熙惠  王燕
作者单位:西安交通大学医学院第一附属医院,儿科,西安,710061
基金项目:陕西省西安市科技局资助项目
摘    要:目的 探讨高压氧(hyperbaric oxygen,HBO)对缺氧缺血性脑损伤(hypoxia-ischemia brain damage,HIBD)新生大鼠远期的学习记忆功能和海马CA1区神经元的保护作用.方法 18只7日龄SD大鼠随机分为3组(n=6),分别是:假手术组、HIBD组、HBO干预组(HBO HIBD).基于Rice法制作HIBD模型,HIBD HBO组大鼠在HIBD后1 h应用2.5 ATA(1 ATA=100 kPa)的高压氧进行一次性治疗,治疗时间为1.5 h.在大鼠6周龄时,应用抑制性回避实验评价其短时程记忆功能,用Morris水迷宫实验评价大鼠8周龄时的空间学习和记忆功能.9周龄时灌注取脑,观察大鼠海马CA1区的神经元死亡和丢失情况.结论 在抑制性回避实验中,各组大鼠在学习期跳台潜伏期差异没有统计学意义,在测试期,HIBD HBO组大鼠的跳台潜伏期长于HIBD组(P<0.05);在Morris水迷宫实验中,HIBD HBO组大鼠无论在定位航行实验中还是在空间探索实验中均比HIBD组表现良好,在定位航行实验中,各组大鼠的寻台潜伏期随着实验次数的增多而逐渐缩短,而HIBD HBO组的平均寻台潜伏期比HIBD组短(P<0.05);在空间探索实验中,无论是站台区探索时间还是穿越站台区的次数,HIBD HBO组均优于HIBD组(P<0.05).HIBD HBO组大鼠的脑组织病理变化比HIBD组轻,且损伤侧的海马CA1区的神经元没有明显的死亡和丢失.结论 新生大鼠HIBD后1 h给予单次高压氧(2.5 ATA,1.5 h)治疗,可以有效地改善大鼠远期的学习记忆功能,同时也可以减轻大鼠海马CA1区的神经元的坏死与丢失.

关 键 词:高压氧  缺氧缺血性脑损伤  学习记忆  海马  高压氧  HIBD  新生大鼠  学习记忆功能  海马  神经元  远期影响  Brain  Damage  Neonatal  Rat  Brain  Injury  坏死  改善  治疗  死亡  损伤  病理变化  脑组织  时间  台区  渐缩
收稿时间:2006-03-07
修稿时间:2006-05-22

Hyperbaric Oxygen Improves Long-term Learning-memory Deficits and Brain Injury in Neonatal Rat with Hypoxia-ischemia Brain Damage
LIU Xiao-hong,ZHAO Yong-li,MA Qiao-mei,ZHOU Xi-hui,WANG Yan.Hyperbaric Oxygen Improves Long-term Learning-memory Deficits and Brain Injury in Neonatal Rat with Hypoxia-ischemia Brain Damage[J].Journal of West China University of Medical Sciences,2007,38(1):73-77.
Authors:LIU Xiao-hong  ZHAO Yong-li  MA Qiao-mei  ZHOU Xi-hui  WANG Yan
Institution:Department of Pediatrics, the First Affiliated Hospital in Xi'an Jiaotong University, Xi'an 710061, China.
Abstract:OBJECTIVE: To investigate the effect of hyperbaric oxygen (HBO) on long-term learning-memory disabilities and brain injury induced by hypoxia-ischemia in neonatal rat. METHODS: In the study, eighteen rats aged seven days were divid into three groups: (1) sham-operated group (SHAM), (2) hypoxia-ischemia group (HIBD), (3) HBO-treated hypoxia-ischemia group (HIBD + HBO). In hypoxia-ischemia groups, left common carotid artery was ligated permanently on the seventh postnatal day, two hours after the procedure; hypoxia (92% nitrogen and 8% oxygen) was induced for 2 h. In HBO-treated hypoxia-ischemia group, single HBO (2. 5 ATA, 1.5 h) was administered at one hour after the hypoxia period. At the six weeks old, step-down inhibitory avoidance test was used to evaluate the short-term memory of rats. Learning and long-term spatial memory deficits were tested using Morris water maze at eight weeks old of rats. Rats were then perfused and brains removed for macroscopic and microscopic evaluation. The cell density of hippocampus were used to evaluate the degree of brain injure. RESULTS: In HIBD+HBO group, the latency to step down the platform was significantly longer than that of HIBD group (P<0.05); in HIBD+HBO group, the mean latencies to reach the platform was significantly shorter than that of HIBD group (P < 0.05); in HIBD + HBO group, the time spent in the target quadrant was significantly lower than that in HIBD group (P<0.05). Histopathological evaluation demonstrated that HBO also significantly diminished brain injury and decreased the cell loss of hippocampal CA1 region. CONCLUSION: Single HBO (2.5 ATA, 1.5 h) can significantly improve long-term learning-memory deficits and attenuate brain injury in rats with hypoxia-ischemia brain damage.
Keywords:Hyperbaric oxygenation Hypoxia-ischemia brain damage Learning-memory Hippocampal CA1 region
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号