ALA-PDT对瘢痕疙瘩成纤维细胞增殖抑制作用的研究

目的 探讨5-氨基酮戊酸光动力疗法(ALA-PDT)在体外对瘢痕疙瘩成纤维细胞增殖的影响,并确定合适的光敏剂浓度和激光能量密度密度.方法 体外培养人体瘢痕疙瘩成纤维细胞.实验分为空白对照组、激光组(仅予不同能量密度He-Ne激光照射)、光敏剂组(仅予不同浓度光敏剂ALA同时培养)和ALA-PDT组(先后予ALA培养和He-Ne激光照射).采用MTT法计算各组细胞增殖抑制率.结果 激光组和光敏剂组对瘢痕疙瘩成纤维细胞增殖无明显抑制作用.ALA-PDT组中,当He-Ne激光剂量(>10 J/cm~2)与ALA浓度(>0.125 mmol/L)达到一定程度时,抑制作用明显,随激光能量密度和药物浓度的同时增大,抑制作用逐渐增大.结论 ALA-PDT对体外培养的人瘢痕疙瘩成纤维细胞增殖有明显抑制作用,其抑制作用随药物浓度和激光能量密度增加而增加,最佳的参数:ALA浓度为0.25 mmol/L,He-Ne激光能量密度为40 J/cm~2.

Study on the Inhibitory Effect of ALA-PDT on the Proliferation of Fibroblasts from Keloid

Objective To investigate the effects of ALA-PDT on the proliferation of fibroblasts from keloid in vitro, and to find the most efficient concentration of photosensitizer and the energy of He-Ne laser light. Methods The fibroblast cells from keloid cultured in vitro were divided into blank control group, laser light group(only with different dose energy of He-Ne laser light), photosensitizer group(only with different concentration of ALA) and ALA-PDT group (treated by photosensitizer and laser light). After the above treatments were finished, the inhibition rate of each group was measured with MTT method. Results There is no obvious inhibitory effect on the fibroblast cells from keloid in laser light group and photosensitizer group, In ALA-PDT groups, when the concentration of ALA and the dose of energy of He-Ne laser light reached some extent(>0.125 mmol/L and >10 J/cm~2 respectively), the inhibitory effect of ALA-PDT kept rising with the increase of the concentration of ALA and the energy of He-Ne laser light. The most appropriate concentration for ALA is 0.25 mmol/L, while the energy for He-Ne laser light is 40 J/cm~2.Conclusion The inhibitory effect of ALA-PDT on the proliferation of fibroblasts from keloid could be enhanced with the increase of photosensitizer concentration and laser light energy.