胎盘生长因子对培养的人脐静脉内皮细胞分泌炎性因子的影响

目的 观察胎盘生长因子(placental growth factor, PlGF)对血管内皮细胞分泌炎性因子的影响.方法 体外培养人脐静脉内皮细胞(human umibilical vein endotheial cells, HUVEC),并采用ELISA技术来观察0～50 ng/mL的重组人PlGF(rhPlGF)作用不同时间(4～48 h)对HUVEC分泌单核细胞趋化因子-1(monocyte chemotactic protein-1, MCP-1)、可溶性血管细胞黏附分子-1(soluble vascular-cell adhesion molecule-1, sVCAM-1)及P-选择素(P-Selectin)功能的变化.结果 rhPlGF对HUVEC分泌MCP-1的刺激作用具有一定的时间及剂量依赖关系,以50 ng/mL的PlGF作用48 h的作用最为明显(P<0.05).与对照组相比,作用于HUVEC 4 h,5～50 ng/mL的PlGF均能明显增加P-Selectin的分泌.但是,不同浓度的rhPlGF处理不同时间,HUVEC分泌sVCAM-1的量与相应时间对照组比较,差异均无统计学意义.结论 PlGF对HUVEC分泌MCP-1及P-Selectin等有明显的促进作用.提示PlGF对内皮细胞分泌炎性因子功能的影响可能很复杂.

Effects of Placental Growth Factor on the Secretion of Proinflammatory Cytochemokines in Vascular Endothelial Cells

Objective Placental growth factor(PlGF)mediates angiogenesis and inflammation,and is up-regulated in early and advanced atherosclerotic lesions.But its effects on the function of vascular endothelial cells is still largely unknown.This study was designed to test the effects of PlGF on the secretion of proinflammatory cytochemokines in vascular endothelial cells.MethodsCultured human umibilical vein endotheial cells(HUVECs)of passages 3-5 were used.Growth-arrested HUVECs were treated with 5,10,25 or 50 ng/mL...