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Aurora A表达对人前列腺癌细胞生物学行为的影响
引用本文:赵凤艳,屈艺,张林,母得志,黄翔,魏大鹏.Aurora A表达对人前列腺癌细胞生物学行为的影响[J].四川大学学报(医学版),2007,38(2):302-305.
作者姓名:赵凤艳  屈艺  张林  母得志  黄翔  魏大鹏
作者单位:1. 四川大学华西基础医学与法医学院,分子生物研究室,成都,610041
2. 四川大学华西第二医院,实验中心
3. 四川省人民医院,泌尿外科
基金项目:国家自然科学基金 , 高等学校博士学科点专项科研项目 , 四川省科技厅资助项目
摘    要:目的 研究Aurora A表达增高对人前列腺癌细胞LNCaP生物学行为的影响,以了解其在人前列腺癌发生发展中的作用.方法 克隆Aurora A全长基因,将其转染入LNCaP细胞中表达,采用四唑盐(MTT)法细胞增殖实验检测Aurora A表达升高的阳性细胞生长情况,采用改良的Transwell侵袭小室方法检测细胞侵袭能力;同时与转染空质粒(pcDNA3.1)细胞和未转染细胞进行比较.结果 成功构建重组质粒pcDNA3.1-Full Length Aurora A.Aurora A高表达的LNCaP细胞增殖速度较空质粒转染细胞和未转染细胞快,培养3 d后LNCaP-Aurora A组细胞较pcDNA3.1质粒转染细胞及未转染的LNCaP细胞增多,差异有统计学意义(P<0.05).在体外细胞移动实验中,LNCaP-Aurora A组细胞也较另两组细胞表现出更强的穿膜能力(P<0.01).结论 Aurora A表达增高与人前列腺癌细胞的恶性表型具有密切相关性,Aurora A在人前列腺癌发生发展中可能发挥重要作用.

关 键 词:Aurora  A  人前列腺癌  增殖  侵袭  Aurora  表达  人前列腺癌  细胞生物学行为  影响  Cells  Prostate  Cancer  Biological  相关性  恶性表型  侵袭能力  表现  实验  细胞移动  统计学意义  差异  培养  增殖速度  Length  重组质粒
收稿时间:2006-04-28
修稿时间:2006-08-16

Effect of Aurora A on Biological Phenotypes of Prostate Cancer Cells
ZHAO Feng-yan,QU Yi,ZHANG Lin,MU De-zhi,HUANG Xiang,WEI Da-peng.Effect of Aurora A on Biological Phenotypes of Prostate Cancer Cells[J].Journal of West China University of Medical Sciences,2007,38(2):302-305.
Authors:ZHAO Feng-yan  QU Yi  ZHANG Lin  MU De-zhi  HUANG Xiang  WEI Da-peng
Institution:Experiment Center, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Abstract:Objective To examine the effect of Aurora A on the biological phenotypes of prostate cancer cells and the role of Aurora A in the carcinogenesis of prostate cancer. Methods Full length Aurora A gene was cloned and transfected into the prostate cancer cell line LNCaP to construct a positive cell clone with high expression of Aurora A. The growth and proliferation of the cells was detected by MTT. The mobility of the cells was examined by transwell cell layer infiltration assay. The growth and motility of LNCaP-Aurora A cells were compared with those of the LNCaP-pcDNA3.1/LNCaP cells. Results Compared to the LNCaP-pcDNA3.1/LNCaP cells, the cells with high expression of Aurora A had faster proliferation and stronger invasive ability. Three days after cultivation, more LNCaP-Aurora A cells had grown than the LNCaP-pcDNA3.1/LNCaP cells (P<0.05). Conclusion The increased expression of Aurora A is closely associated with the malignant phenotypes of prostate cancer. Aurora A may play a very important role in the carcinogenesis of prostate cancer.
Keywords:Aurora A  Human prostate cancer  Proliferation  Mobility
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