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部分背根切断及针刺促进备用背根节c-jun的表达
引用本文:柯青,王廷华,李朗,于建云,郭泽云.部分背根切断及针刺促进备用背根节c-jun的表达[J].四川大学学报(医学版),2003,34(2):248-250.
作者姓名:柯青  王廷华  李朗  于建云  郭泽云
作者单位:昆明医学院,神经科学研究所,昆明,650031
基金项目:国家自然科学基金 (批准号 3 0 0 0 0 2 2 7),云南省教委基金( No.0 0 12 0 5 1)资助
摘    要:目的 探讨部分背根切断术及针刺对背根节(DRG)c-jun表达的影响。方法 针成年雄猫15只,分假手术组,手术组和术后针刺组,每组5只。手术组动物行单侧备用根术,术后针刺组动物经单侧备用根术后,针刺后肢手术侧L6脊神经分布区中的两组穴位,足三里和悬中:伏兔和三阴交,每天一次,每次30min,连续针刺7d,通过免疫组化ABC法,用c-jun特异抗体(1:1000,Santa Cruz)对切片进行染色,DAB呈色。观察并测量DRG中c-jun阳性神经元的分布,数量及反应强度。结果 在假手术组DRG中,只有部分神经元染色。手术组c-jun阳性神经元的数值较假手术组显著增多(P<0.05),其免疫反应水平较假手术组,显著上升(P<0.05)。术后针刺组较手术组的c-jun免疫反应强度增强,而阳性神经元的数量没有显著差异。结论 部分背根切断后备用DRG神经元中c-jun的表达增加,针刺促使c-jun的表达进一步上调。

关 键 词:c-jun表达  背根节  部分背根切断术  针刺
修稿时间:2002年6月10日

c-jun Expression in Spared Dorsal Root Ganglion Following Partial Dorsal Root Rhizotomy and Acupuncture
Ke Qing,Wang Tinghua,Li Lang,Yu Jianyun,Guo Zeyun. Research Institute of Neuroscience,Kunming Medical College,Kunming ,China.c-jun Expression in Spared Dorsal Root Ganglion Following Partial Dorsal Root Rhizotomy and Acupuncture[J].Journal of West China University of Medical Sciences,2003,34(2):248-250.
Authors:Ke Qing  Wang Tinghua  Li Lang  Yu Jianyun  Guo Zeyun Research Institute of Neuroscience  Kunming Medical College  Kunming  China
Institution:Research Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
Abstract:OBJECTIVE: To investigate the change of c-jun expression in spared dorsal root ganglion (DRG) following partial dorsal root rhizotomy and acupuncture. METHODS: Fifteen adult cats were divided into three groups with five cats in every group--sham operation, operation, and acupuncture following operation respectively. Animals of operation group were subjected to unilateral root rhizotomy (L1-L5, L7-S2 DRGs were sectioned, L6 DRG was spared). Electro-needling was performed alternatively at two sets of acupoints, including Zusanli and Xuanzhong, Futu and Sanyinjiao, located in the distribution area of spinal nerve L6 on the operated side, 30 min everyday for 7 days following unilateral root rhizotomy. The animals of sham operation group experienced only skin and muscle incisions. On the 7th day after operation, all animals were sacrificed. The L6 DRG on the disposal side of each animal was taken and made into frozen sections 20 micrograms in thickness. The sections were stained under the same condition using specific c-jun antibody (1:1000, Santa Cruz) by immunohistochemistry ABC method. The distribution and number as well as immunoreactive level of c-jun positive neurons in DRG were observed and measured. RESULTS: In the L6 DRG of sham operation group, only parts of neurons were stained. In contrast, the number of positive neurons and immunoreactive level for c-jun increased significantly in operation group (P < 0.05). Compared with the operation group, the operation plus acupuncture group had higher c-jun immunoreactive level of neurouns, but its number of c-jun positive neurons didn't change markedly. CONCLUSION: Partial dorsal root rhizotomy leads to the up-regulation of c-jun expression in neurons of spared DRG, and acupuncture enhances the up-regulation.
Keywords:c  jun expression    Dorsal root ganglion    Partial dorsal root rhizotomy    Acupuncture
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