D4S1647、D6S2414基因座在中国汉族、蒙古族、藏族的遗传多态性

目的 调查D4S1647、D6S2414基因座在中国汉族、蒙古族、藏族群体中的遗传分布规律。方法 采集308份血及唾液标本应用PCR技术，扩增产物用非变性聚丙酰胺凝胶电泳分离，银染显色分析。结果 两位点各群体基因型频率分布均符合Hardy-Weinberg平衡，每一位点等位基因频率分布在各群体间无显著差异；通过对10个汉族家系的遗传模式分析，证实了两位点等位基因传递遵循孟德尔遗传规律。结论 D4S1647、D6S2414基因座在中国汉族，蒙古族，藏族群体均具有高度遗传多态性。

Sequence Analysis and Genetic Polymorphisms of D4S1647 and D6S2414 Loci in Chinese Han,Mongolian and Tibetan Populations

Objective To investigate the polymorphisms of two short tandem repeats (STR) loci D4S1647 and D6S2414 in Chinese Han,Mongolian and Tibetan populations. Methods Blood and saliva samples were collected from 308 unrelated individuals in the three populations. DNA was extracted by chelex 100 method and amplified by the polymerase chain reaction (PCR). The PCR products were analyzed by nondenaturing polyacrylamide gel electrophoresis and silver staining. Results The genotype distributions of D4S1647 and D6S2414 loci were in accordance with Hardy Weinberg equilibrium.The frequencies of alleles showed no significant differences among the three populations. Analyses of Han families indicated that the alleles agreed with Mendelian inheritance law. Conclusion This study yielded information on the genetic polymorphisms of the two STR loci in Chinese Han,Mongolian and Tibetan populations.