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空肠/结肠弯曲菌的生物分型、血清学分型和质粒分析
引用本文:吕德生,陈志新,王伯伦,徐荷.空肠/结肠弯曲菌的生物分型、血清学分型和质粒分析[J].四川大学学报(医学版),1988(1).
作者姓名:吕德生  陈志新  王伯伦  徐荷
作者单位:华西医科大学流行病学教研室 (吕德生,陈志新,王伯伦),华西医科大学流行病学教研室(徐荷)
摘    要:本文对来自成都地区的空肠/结肠弯曲菌同时进行了生物分型、血清学分型和质粒分析,对这三种方法的相互关系及其作用进行了探讨,发现同一血清型的弯曲菌中,其生物型和质粒谱可完全不同,提示生物分型和质粒分析在一定程度上可弥补血清学分型的不足。同时,注意到结肠弯曲菌的质粒检出率明显地高于空肠弯曲菌,这对于弯曲菌上质粒的进一步研究具有重要意义。

关 键 词:空肠/结肠弯曲菌  生物分型  血清学分型  质粒分析

BIOTYPING AND SEROTYPING AND PLASMID ANALYSIS OF CAMPYLOBACTER JEJUNI/COLI
Lv Desheng,Chen Zhixin,Wang Bolun,Xu He.BIOTYPING AND SEROTYPING AND PLASMID ANALYSIS OF CAMPYLOBACTER JEJUNI/COLI[J].Journal of West China University of Medical Sciences,1988(1).
Authors:Lv Desheng  Chen Zhixin  Wang Bolun  Xu He
Institution:Lv Desheng;Chen Zhixin;Wang Bolun;Xu He Department of Epidemiology
Abstract:Biotyping, serotyping and plasmid a-nalysis of 78 Campylobacter jujuni/coli weremade. These isolates were obtained fromchildren with acute diarrhea, healthy car-riers and animals (chickens, pigs, dogs.cats and geese) in Chengdu district. Ofthe 78 strains, 14 possessed plasmid DNA.Plasmid occurrence rate was 17.95%. Moststrains that carried plasmids had between2 and 3 of them. The incidence of plasmidsobserved in Campylobacter coli strains(33.3%) was significantly greater than thatobserved in Campylobacter jejuni strains(9.8%). The study shows that biotypesand plasmid profiles of Campylobacter mayentirely be different in the same serotype.It is suggested that biotyping and plasmidanalysis may be used as a supplement toserotyping.
Keywords:Campylobacter jejuni/coli  Biotyping  Serotyping  Plasmid analysis
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