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应用组织芯片技术研究A103和Inhibin α在肾上腺皮质腺瘤中的表达
引用本文:张红英,刘卫平,王晓洁,李甘地,郭嘉,李俸媛,廖殿英.应用组织芯片技术研究A103和Inhibin α在肾上腺皮质腺瘤中的表达[J].四川大学学报(医学版),2003,34(3):424-426.
作者姓名:张红英  刘卫平  王晓洁  李甘地  郭嘉  李俸媛  廖殿英
作者单位:四川大学华西医院,病理科,成都,610041
基金项目:四川省科委重点项目(01SG013-03)和纽约中华医学基金会(00-722)资助
摘    要:目的:研究A103及Inhibinα在肾上腺皮质腺瘤中的潜在诊断价值并探讨组织芯片技术应用的可行性.方法:制备肾上腺组织芯片,含79例样本,其中正常肾上腺3例,肾上腺皮质腺瘤66例,肾上腺转移癌5例,嗜铬细胞瘤5例。用免疫组织化学Envision法检测A103及Inhibinα的表达。结果:免疫组织化学染色A103阳性率分别为正常肾上腺皮质100%(3/3),肾上腺皮质腺瘤90.2%(55/61),肾上腺转移癌0%(0/4),嗜铬细胞瘤0%(O/4);Inhibinα在正常肾上腺皮质中100%表达,阳性部位主要在网状带与束状带内层,肾上腺皮质腺瘤阳性率83.6%(51/61),肾上腺转移癌O%(O/4),嗜铬细胞瘤0%(0/4).结论:A103及Inhibinα的联合应用对于肾上腺皮质腺瘤的诊断及其与其它肿瘤的鉴别诊断有较高价值。

关 键 词:组织芯片技术  研究  A103  Inhibinα  肾上腺皮质腺瘤  表达
修稿时间:2002年9月10日

The Expression of A103 and Inhibin α in Adrenocortical Adenoma by High-throughput Tissue Microarray Techniques
Zhang Hongying,Liu Weiping,Wang Xiaojie,Li Gandi,Guo Jia,Li Fengyuan,Liao Dianying.The Expression of A103 and Inhibin α in Adrenocortical Adenoma by High-throughput Tissue Microarray Techniques[J].Journal of West China University of Medical Sciences,2003,34(3):424-426.
Authors:Zhang Hongying  Liu Weiping  Wang Xiaojie  Li Gandi  Guo Jia  Li Fengyuan  Liao Dianying
Institution:Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
Abstract:OBJECTIVE: To evaluate the potential diagnostic role of A103 and inhibin alpha in adrenocortical adenoma and the feasibility of tissue microarray/tissue chips. METHODS: A series of adrenal gland tissue chips were prepared, including 79 cases of formalin-fixed, paraffin-embedded tissue from 3 normal adrenal glands, 66 adrenocortical adenomas, 5 cases of metastatic carcinoma and 5 pheochromocytomas. A103 and inhibin alpha were detected with Envision immunohistochemical staining methods. RESULTS: Positive staining was seen in 3 of 3 (100%) adrenal cortices and in 55 of 61(90.2%) adrenocortical adenomas but in none of the pheochromocytomas or adrenal metastatic carcinomas. In the 3 of 3(100%) adrenal cortices, inhibin alpha immunoreactivity was limited to the zona reticularis and the innermost zona fasciculata. 51 of 61(83.6%) adrenocortical adenomas reacted with inhibin alpha. None of the pheochromocytomas or metastatic carcinomas reacted with inhibin alpha. CONCLUSIONS: High-throughput tissue microarray/tissue chip offers a powerful strategy for rapid identification in situ. It is feasible to utilize tissue microarray for a rapid, economic and accurate screening of tissue specimens on a large scale. The combined use of A103 and inhibin alpha is valuable in distinguishing adrenocortical adenoma from pheochromocytoma and other metastatic carcinomas.
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