成都地区汉族人群X染色体3个STR基因座的遗传多态性及其法医学应用

目的了解X染色体3个STR基因座在中国成都汉族群体的遗传多态性及其法医学应用价值。方法利用PCR和非变性聚丙烯酰胺凝胶电泳对中国成都地区无亲缘关系汉族个体X染色体3个STR基因座进行分型，自动激光荧光测序仪测定DNA序列，并检验女性基因型频率分布是否符合Hardy—Weinberg平衡，计算法医学常用各种概率。结果3个STR基因座在成都地区汉族群体均具有遗传多态性，X^2检验表明女性的基因型频率分布符合Hardy—Weinberg平衡。结论为人类群体研究提供了X染色体3个STR基因座的中国成都汉族群体数据，并为应用这些遗传标记进行亲子鉴定和个人识别提供了概率计算依据。

Genetic Polymorphisms of Three STR Loci on Chromosome X and Their Forensic Application in a Chinese Han Population

Objective To understand the allele structure and genetic polymorphism at three STR loci on chromosome X in Chinese Han population and make an evalution of their forensic application. Methods EDTA blood samples were collected from the unrelated individuals in Chengdu , China. After being extracted with Chelex method, the DNA samples were amplified by PCR technique. The PCR products were analyzed by PAG electrophoresis and the approach of automated fluorescence detection. Hardy Weinberg equilibrium of females was tested and every forensic interested value was calculated. Results The polymorphisms of all 3 STR loci were obtained from 100 unrelated females and 120 unrelated males from Chinese Han ethnic group. Chi square tests on the genotype frequencies in females did not reveal deviations from Hardy Weinberg equilibrium. Conclusion The obtained data are beneficial to understanding the population genetics of the three STR loci in Chinese Han population. For forensic genetics, the obtained data can be used to calculate the probabilities dealing with the paternity test and the individual identification.