黄连素对缺血再灌注心肌细胞损伤的保护作用

目的　研究黄连素对新生大鼠心肌细胞缺血再灌注损伤的保护作用。方法　取体外培养的新生大鼠心肌细胞于缺氧 2 4h复氧 1h造成缺血再灌注 ( I/ R)模型 ,观察细胞损伤情况 ;并将黄连素以 1.5× 10 - 6 m ol/ L、1.5× 10 - 5m ol/ L、1.5× 10 - 4 mol/ L 三种浓度分别加入培养基中 ,预处理 2 4h后 ,再置于上述缺氧复氧环境中培养 ,检测以上不同条件下细胞上清液中的乳酸脱氢酶 ( L DH)、丙二醛 ( MDA)、超氧化物歧化酶 ( SOD)含量 ,并检测各组细胞的凋亡率。结果　与正常对照组相比 ,缺血及再灌组细胞上清液中 L DH、MDA含量明显升高 ( P<0 .0 1) ,SOD活力则显著降低 ( P<0 .0 1) ,缺血组和再灌组凋亡率均升高明显 ( P<0 .0 1)。而用黄连素预处理后缺血及再灌组的 L DH、MDA显著低于用药前 ( P<0 .0 1) ,SOD则高于单纯缺血和再灌组 ( P<0 .0 1) ,上述作用在本实验黄连素浓度为 1.5× 10 - 6 m ol/ L～ 1.5× 10 - 4 mol/ L 范围内 ,随浓度升高而更加明显。特别是用黄连素 1.5× 10 - 5mol/ L 浓度预处理后 ,缺血组和再灌组的细胞凋亡率分别是 14.4%和 2 0 % ,分别与用药前 ( 17.4%和 41% )比较有显著性差异 ( P<0 .0 1)。结论　黄连素对缺血再灌心肌细胞有保护作用 ,其作用与浓度有一定依赖关系

Protective Effect of Berberine on Cardiac Myocyte Injured by Ischemia-Reperfusion

OBJECTIVE: To observe the protective effect of berberine on cardiac myocyte injured by ischemia-reperfusion(I/R) in neonatal rats. METHODS: Cardiac myocytes from neonatal SD rat were cultured and pretreated with berberine in three different concentrations, 1.5 x 10(-6) mol/L, 1.5 x 10(-5) mol/L and 1.5 x 10(-4) mol/L, before exposure to hypoxia (95% N2-5% CO2) for 24 h and reoxygenation (95% air-5% CO2) for 1 h to create cell model of ischemia-reperfusion. Then lactate dehydrogenase (LDH) release, methylenedioxyamphetamine (MDA) release superoxide dismutase (SOD) activity were measured, and cell apoptosis was detected. RESULTS: Compared with the measurements taken in normal group, the LDH and MDA in the supernatant of the cells in ischemia and reperfusion group increased highly (P < 0.01), SOD activity decreased sharply (P < 0.01), and apoptosis of cells in ischemia groups and reperfusion groups increased highly (P < 0.01). Pretreatment of myocytes with berberine resulted in reduction in LDH and MDA release (P < 0.01) in I/R groups, attenuation of apoptosis in ischemia and reperfusion groups (P < 0.01). When the concentration of berberine was increased, these effects were getting obvious. Especially when the myocytes were pretreated with berberine at 1.5 x 10(-5) mmol/L, the cell apoptosis rates were 14.4% and 20% in ischemia group and reperfusion group, which were lower than the rates of 17.4% and 41% before pretreatment, respectively. CONCLUSION: Berberine alleviates I/R injury and attenuates apoptosis in myocytes exposed to I/R. These effects partly depend on the concentration of berberine.