| 中国两种地方猪种内源性逆转录病毒的亚型分析 |
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| 作者姓名： | Zhang L Bu H Li S Yu P Li Y Cheng J |
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| 作者单位： | 1. 四川大学华西医院,卫生部移植工程与移植免疫重点实验室,成都,610041
2. 四川大学华西医院,病理科 |
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| 基金项目： | 国家自然科学基金 (批准号 3 0 0 70 72 5 )资助 |
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| 摘 要： | 目的 对两种中国地方猪种（五指山猪和版纳微型猪近交系）的86头个体进行猪内源性逆转录病毒（PERV）亚型分析。方法 取引物Env A,EnvB和EnvC分别用于鉴定PERV的A，B和C亚型，并对86头个体进行PCR法分析。结果 在所有被检测个体中，77．9％的个体的基因组中 时有PERV的A、B两种亚型，另外22．1％的个体的基因组中则只有PERV的A或B亚型；所有个体的外周血白细胞中均未检测到PERV的C亚型。结论 PERV在中国五指山猪和版纳微型猪近交系的外周血白细胞中只有A、B两种亚型，并且以AB的混合亚型为主。
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| 关 键 词： | 异种移植 人－兽共患病 猪内源性逆转录病毒 病毒亚型 |
| 修稿时间： | 2002年5月10日 |
Analysis of subtype of porcine endogenous retrovirus in two species of Chinese pigs |
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| Zhang L,Bu H,Li S,Yu P,Li Y,Cheng J.Analysis of subtype of porcine endogenous retrovirus in two species of Chinese pigs[J].Journal of West China University of Medical Sciences,2003,34(2):197-199. |
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| Authors: | Zhang Li Bu Hong Li Shengfu Yu Ping Li Youping Cheng Jingqiu |
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| Institution: | Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China. |
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| Abstract: | Objective To analyze the subtype of porcine endogenous retrovirus (PERV) in two species of Chinese pigs Wu zhishan pig and Banna minipig inbred; the total number of pigs being eighty six. Methods Three primers which are specific for the three PERV subtypes (subtypes A,B and C) were used to amplify the genome DNA extracted from the pigs. Results In all tested pigs' genome, subtype C was not found; in 77.9% pigs, double positive envAB were found; in 22.1% pigs, only A or B was found. Conclusion In the genome of peripheral leukocytes from Wuzhishan pig and Banna minipig inbred, only subtypes A and B exist, and envAB predominate. |
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| Keywords: | Xenotransplantation Xenozoonosis Porcine endogenous retrovirus Subtype |
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