槲皮素对人乳腺癌裸鼠移植瘤细胞增殖和凋亡的影响

目的　观察槲皮素 ( Quercetin)对人乳腺癌裸鼠移植瘤的抑制作用及其对肿瘤细胞增殖和凋亡的影响。方法　以 MCF- 7细胞复制裸鼠异种移植瘤模型 ,2 4只荷瘤鼠随机分组用药 :对照组、槲皮素组、5 - Fu组、槲皮素 + 5 - Fu组。用药 15 d收集肿瘤标本 ,采用光镜、电镜观察 ,Tunel法行原位凋亡检测 ,免疫组化分析 Ki67、Bcl- 2的表达。结果　 1槲皮素组、5 - Fu组、槲皮素 + 5 - Fu组瘤重明显低于对照组 ( P<0 .0 5 ) ;2 Ki67免疫组化显示 :槲皮素组、5 - Fu组、槲皮素 + 5 - Fu组 Ki67指数 ( Ki67- L I)明显低于对照组 ,差异有显著性 ( P<0 .0 1) ;Bcl- 2免疫组化显示 :槲皮素组、5 - Fu组、槲皮素 + 5 - Fu组与对照组差异有显著性 ( P<0 .0 5 ) ;3原位凋亡检测显示 :槲皮素组、5 -Fu组、槲皮素 + 5 - Fu组凋亡指数 ( AI)高于对照组 ( P<0 .0 1) ,槲皮素 + 5 - Fu组高于槲皮素组、5 - Fu组 ,差异有显著性意义 ( P<0 .0 5 )。结论　槲皮素可抑制人乳腺癌细胞 MCF- 7移植瘤生长

Effects of Quercetin on the Proliferation and Apoptosis in Transplantation Tumor of Breast Cancer in Nude Mice

OBJECTIVE: To investigate the effects of quercetin on tumour growth, cell proliferation and apoptosis in transplantation tumor of breast cancer cell line MCF-7 in nude mice. METHODS: MCF-7 cells were inoculated into the mammary fatty pad of nude mice to establish breast cancer model, then twenty-four BALB/c nude mice with xenograft tumor were randomized into four groups: Control group, Quercetin group, 5-Fu group and Quercetin + 5-Fu group. After 15 days treatment, samples of tumor were collected. The sections of tumor were observed under light microscope and electron microscope. Cell apoptosis in situ was examined by a Tunel assay, and the expressions of ki67 antigen and B-cell lymphoma/leukemia-2 (Bcl-2) were detected by immunohistochemistry. RESULTS: 1. The tumor weight of Control group was significantly higher than those of Quercetin group, 5-Fu group and Quercetin + 5-Fu group (P < 0.05). 2. Immunohistochemical staining of ki67 showed that the ki67 label index (ki67-LI) displayed significant difference between Control group and Quercetin group, 5-Fu group, Quercetin + 5-Fu group, and so did the staining of Bcl-2. 3. Detection of apoptosis in situ showed that apoptosis index (AI) was significantly higher in Quercetin group, 5-Fu group and Quercetin + 5-Fu group than in control group (P < 0.01), and higher AI was observed in Quercetin + 5-Fu group as compared with the AI in Quercetin group and 5-Fu group (P < 0.05). CONCLUSION: Quercetin can inhibit the growth of transplantation tumor of breast cancer cell line MCF-7 in nude mice.