EB病毒LMP1基因和蛋白在结外鼻型NK/T细胞淋巴瘤中的表达及与预后的关系

目的从蛋白和DNA两个水平初步检测成都地区结外鼻型NK／T细胞淋巴瘤中LMP1的表达，并探讨与预后的关系。方法应用免疫组化和PCR技术检测67例结外鼻型NK／T细胞淋巴瘤LMPl的表达，并应用Kaplan—Meier曲线分别比较LMP1蛋白和LMP1DNA阳性表达组与阴性表达组的生存率。结果LMP1蛋白阳性表达10例(14．93％)，LMP1DNA阳性表达56例(83．58％)，LMP1总检出率83．58％。LMP1蛋白(P=0．678)和LMP1DNA(P=0．943)表达均与预后无明显关系。结论LMP1与成都地区结外鼻型NK／T细胞淋巴瘤关系密切；结外鼻型NK／T细胞淋巴瘤中LMP1蛋白水平和DNA水平表达不一致；LMP1的表达与预后无明显关系。

Extranodal Nasal Type NK/T-cell Lymphoma: the Expression of Epstein-Barr Virus Latent Membrane Protein 1 and Its Significance of Prognosis

Objective To investigate the expression and prognostic significance of Epstein-Barr virus latent membrane protein 1 in extranodal nasal type NK/T-cell lymphoma in the Chengdu area. Methods The expression of latent membrane protein-1(LMP1) was detected by immunohistochemistry (IHC) and DNA-PCR in 67 cases of extranodal nasal type NK/T-cell lymphoma, and the differences in survival rate between positive and negative expression groups of LMP1-protien and LMP1-DNA were analyzed respectively. Results Ten(14.93%)cases were positive at LMP1-protein level, and fifty-six(83.58%)were positive at LMP1-DNA level. The total expression rate of LMP1 was 83.58%. No statistically significant difference was observed between the expression of LMP1 and prognosis(P=0.678) and between the expression of LMP1-DNA and prognosis (P=0.943). Conclusion LMP1 was shown to be closely associated with extranodal nasal type NK/T-cell lymphoma in Chengdu. The expression rate of LMP1 at protein level was different from that at DNA level. No relationship was found between the prognosis and the LMP1 expression in extranodal nasal type NK/T-cell lymphoma.