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辣椒素对大鼠心肌缺血再灌注损伤保护作用的研究
引用本文:秦素兰,刘陕岭,王儒蓉.辣椒素对大鼠心肌缺血再灌注损伤保护作用的研究[J].四川大学学报(医学版),2008,39(4).
作者姓名:秦素兰  刘陕岭  王儒蓉
作者单位:1. 四川大学华西医院麻醉科,成都,610041;四川省广安市人民医院麻醉科
2. 四川大学华西医院麻醉科,成都,610041
摘    要:目的研究辣椒素(capsaicin)是否对在体大鼠局部心肌缺血再灌注损伤有保护作用及其可能的机制。方法采用结扎大鼠心脏左冠状动脉前降支45min、再灌注120min建立局部心肌缺血再灌注损伤模型。将40只成年健康雄性大鼠随机分为4组:对照组、辣椒素组、辣椒平组、S-3144(P物质受体拮抗剂)组,所有用药分别于缺血前10min和5min经右颈总动脉单次注射入左心室。连续监测心电图及左室功能变化,测定血清磷酸肌酸激酶同工酶(CKMB)浓度,Evans-blue-TTC双染色法测定心肌梗死面积。结果组内比较,缺血再灌注期各时点左室发展压(LVDP)均比结扎前低(P<0.05);除辣椒素组外,各组内再灌注120min时心率比再灌注前各时点均低(P<0.05)。组间比较,辣椒素组再灌注120min时心率明显高于其他3组(P<0.05),再灌注60min、120min时LVDP高于其他3组(P<0.05);实验终点时,辣椒素组CKMB浓度明显低于其他3组(P<0.05),心肌梗死面积小于其他3组(P<0.05);辣椒平组和S-3144组CKMB浓度、心肌梗死面积与对照组比较差异无统计学意义(P>0.05)。结论辣椒素对在体大鼠心肌缺血再灌注损伤具有保护作用,且可能是通过激活辣椒素受体(TRPV1)引起P物质释放,进一步激活P物质受体起作用。

关 键 词:辣椒素  辣椒素受体  P物质  缺血再灌注损伤

Protective Effect of Capsaicin on Against Myocardial lschemia-reperfusion Injury of Rat in vivo
QIN Su-lan,LIU Shan-ling,WANG Ru-rong.Protective Effect of Capsaicin on Against Myocardial lschemia-reperfusion Injury of Rat in vivo[J].Journal of West China University of Medical Sciences,2008,39(4).
Authors:QIN Su-lan  LIU Shan-ling  WANG Ru-rong
Abstract:Objective To explore whether the capsaicin offers protective effect and possible mechanism on against myocardial ischemia-reperfusion injury of rat in vivo.Methods Ligating the left anterior descending coronary artery for 45 min and loosing it for 120 min were performed to establish the rat model of local myocardial ischemia-reperfusion injury in vivo.Forty healthy male rats were randomLy divided into control group,capsaicin group,capsazepine group,and S-3144 group.In 10 min and 5 min before ischemia,all drugs for this study were delivered into rat left ventricle(LV)via right carotid artery.Variations of left ventricular function were successively monitored with ECG(electrocardiogram).Levels of creatine kinase MB(CKMB)in serum were determined.Evans-blue and TTC were utilized to identify the area at risk and the infarct size of LV.Results In all groups,the variables of left ventricular developed pressure(LVDP)significantly decreased after ischemia and reperfusion(P<0.05).Except capsaicin group,heart rates(HR)of three other groups markedly reduced with reperfusion for 120 min as compared to pre-reperfusion(P<0.05).In capsaicin group,there were significant increases in HR and LVDP with reperfusion for 120 min when compared with the other three groups(P<0.05).At the end of experiment,the levels of CKMB and infract size were lower in capsaicin group than in the other three groups(P<0.05),and there was no statistical significance among the other three groups(P>0.05).Conclusion Pretreatment with capsaicin can attenuate myocardial ischemia-reperfusion injury,of which the likely mechanism is by stimulating capsaicin receptor or TRPV1 and further activating substance P receptor in the rat in vivo.
Keywords:Capsaicin TRPV1 Substance P Ischemia-reperfusion injury
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