miR-130a在卵巢癌顺铂耐药细胞株中的表达及其意义

目的分析miR-130a在卵巢癌顺铂耐药细胞株中的表达,探讨miR-130a与卵巢癌对顺铂耐药的关系。方法以浓度梯度递增法构建卵巢癌耐顺铂细胞株,MTT法对耐药细胞株进行鉴定并测定耐药指数,应用荧光实时定量PCR检测并分析各细胞株中miR-130a的表达。结果成功构建卵巢癌顺铂耐药细胞株A2780/CIS1、A2780/CIS2和SKOV3/CIS(P<0.05),其耐药指数分别为30.2、5.3和24.5。荧光实时定量PCR结果显示miR-130a在卵巢癌耐药细胞株中存在稳定的异常高表达(P<0.05),A2780/CIS1、A2780/CIS2以及SKOV3/CIS中miR-130a表达分别较亲本细胞平均上调30.51倍、4.87倍和24.43倍。各耐药细胞中miR-130a表达上调倍数接近于各自的顺铂耐药指数。结论 miR-130a表达的上调可能与卵巢癌细胞株对顺铂的耐药性密切相关。推测抑制miR-130a的表达有助于逆转卵巢癌顺铂耐药性,miR-130a有望成为逆转卵巢癌顺铂耐药性的潜在基因治疗靶点。

Expression of miR-130a in Cisplatin Resistant Cell Lines of Ovarian Cancer

Objective To determine the expression of miR-130a in cisplatin resistant cell lines of ovarian cancer and its impact on cisplatin resistance.Methods Cisplatin resistant ovarian cancer cell lines were established by stepwise selection with gradual increase of cisplatin.MTT assay was applied to indentify the cisplatin resistant cell lines and determine their resistance index.The expression of miR-130a was measured by SYBR green real-time PCR.Results The resistance index of A2780/CIS1,A2780/CIS2 and SKOV3/CIS was 30.2,5.3 and 24.5 respectively.The SYBR green real-time PCR showed that miR-130a was over-expressed in all of the cisplatin resistant cell lines(P<0.05).The expression of miR-130a was 30.51 times higher in A2780/CIS1,4.87 times higher in A2780/CIS2 and 24.43 times higher in SKOV3/CIS than in their parental cell lines(P<0.05),which was almost equally reflected in their resistance index.Conclusion The over expression of miR-130a is associated with cisplatin resistance of ovarian cancer.Inhibiting miR-130a expression may help reverse the cisplatin resistance of ovarian cancer.miR-130a is expected to be a new potential target of genetic therapy for cisplatin resistant ovarian cancer.