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黄芪诱导大鼠骨髓间充质干细胞分化为神经样细胞的研究
引用本文:董立华,王勇,陆长青,王凡.黄芪诱导大鼠骨髓间充质干细胞分化为神经样细胞的研究[J].四川大学学报(医学版),2007,38(3):417-420.
作者姓名:董立华  王勇  陆长青  王凡
作者单位:四川大学华西基础医学与法医学院,人体解剖学教研室,成都,610041
摘    要:目的 研究大鼠骨髓间充质干细胞(MSCs)体外黄芪诱导分化为神经元样细胞.方法 通过密度梯度离心从大鼠骨髓中分离有核细胞进行培养,观察细胞的形态和生长情况,分离纯化,传代扩增.采用含黄芪的无血清L-DMEM诱导分化为神经元样细胞,观察细胞形态变化,以免疫组织化学染色方法检测分化细胞中巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)的表达.用RT-PCR方法半定量分析相关基因neurogenin-1(Ngn-1)和Wnt-1基因在诱导过程中的表达.结果 经传代后,骨髓间充质干细胞呈纤维细胞样,有较强的增殖能力.经黄芪诱导后,细胞形态发生改变,nestin、NSE和GFAP表达阳性,分化为神经元或胶质细胞样细胞.Ngn-1和Wnt-1基因在黄芪诱导后明显上调.结论 大鼠骨髓间充质干细胞可以体外分离、培养,并在黄芪诱导下向神经元样细胞分化.Ngn-1和Wnt-1基因在其分化过程中起正调控作用.

关 键 词:骨髓间充质干细胞  黄芪  诱导分化  黄芪诱导  大鼠骨髓  间充质干  细胞分化  神经样细胞  研究  Mesenchymal  Stem  Cells  Bone  Inducing  Astragalus  调控作用  分化过程  体外分离  胶质细胞  表达阳性  形态发生  增殖能力  纤维细胞  结果  相关基因
收稿时间:2006-08-31
修稿时间:2006-11-02

Effect of Astragalus mongholicus on Inducing Differentiations of Rat Bone Marrow-derived Mesenchymal Stem Cells into Neurocyte-like Cells
DONG Li-hua,WANG Yong,LU Chang-qing,WANG Fan.Effect of Astragalus mongholicus on Inducing Differentiations of Rat Bone Marrow-derived Mesenchymal Stem Cells into Neurocyte-like Cells[J].Journal of West China University of Medical Sciences,2007,38(3):417-420.
Authors:DONG Li-hua  WANG Yong  LU Chang-qing  WANG Fan
Institution:Departerment of Anatomy, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Abstract:Objective To investigate the differentiations of rat bone marrow-derived mesenchymal stem cells (MSCs) into neurocyte-like cells induced by Astragalus mongholicus. Methods We isolated the nucleated cells from rat bone marrows through gradient centrifugation, and cultured and purified the MSCs. The non-serum-L-DMEM containing Astragalus mongholicus was used to induce the differentiations of the MSCs into neurcyte-like cells. The morphologic changes of the differentiated cells were observed. The expressions of the nestin, neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP) were detected by immuncytochemistrical staining. The expressions of the Wnt-1 gene and neurogenin-1(Ngn-1)gene were detected by semi-quantitative RT-PCR. Results The subculturing cells were fibroblast-like, with higher reproductive activities than the primary cells. The Astragalus mongholicus induced morphology changes of the MSCs. The immunocytochemistrical staining of Nestin, NSE and GFAP were positive. The MSCs became neuron-like and gliocyte-like cells. The Astragalus mongholicus also enhanced the expression of Wnt-1 gene and Ngn-1 gene. Conclusion The MSCs can be isolated and cultured in vitro. and can differentiate into neurocyte-like and gliocyte-like cells. Wnt-1 gene and Ngn-1 gene play important regulatory roles during the differentiation of the rat bone marrow-derived mesenchymal stem cells to neurocyte-like cells.
Keywords:Bone marrow-derived mesenchymal stem cells Astragalus mongholicus Differentiation
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