健康大鼠服用益肾通络方后血清中阿魏酸的RP-HPLC测定

目的:建立高效液相色谱(HPLC)法测定健康大鼠血清中阿魏酸的血药浓度。方法:采用益肾通络方浸膏水溶液健康大鼠灌胃,每日2次,连续3天,制备含中药大鼠血清。以反相(RP)-HPLC法测定大鼠含药血清,采用Kromasil C18为色谱柱,0.2%甲醇乙腈-0.2%磷酸和0.2%三乙胺水溶液(18∶82)为流动相,流速0.8mL/nin,检测波长322nm。结果:阿魏酸在10.25-162.00ng/mL与峰面积线性关系良好(r=0.9916),日内与日间精密度的RSD均小于5%,平均回收率为99.53%。结论:该法稳定、快速、灵敏,精密度好,适用于大鼠血清中阿魏酸的含量测定。

Determination of Ferulic Acid in Serum of Yishentongluo Decoction by RP-HPLC in Healthy Rats

Objective To develop an HPLC method for determination of ferulic acid concentrated on the serum of Yishentongluo decoction in healthy rats.Methods Preparation of healthy rats serum contained Chinese herbal formula by intragastric administrating Yishentongluo water-extract,twice per day,continuating 3 days.The determination was carried out with RP-HPLC,using Kromasil-C18 column,0.2% methanol in acetonitrile-0.2% orthophosphoric acid and 0.2% triethylamine water solution(18∶82) as mobile phase with a flow rate of 0.8mL/min and at the detection wavelength of 322 nm.Results The calibration curve showed a good linearity over the range of 10.25-162.00ng/mL(r=0.9916).RSD was less than 5% within day and day-to-day,the average recovery was 99.53%.Conclusion This method was stable,rapid,sensitive and exact.It was appropriate for the quantitative determination of ferulic acid in the rats serum.