丁苯酞对Wistar大鼠血管性痴呆模型中海马区OX-42的影响 |
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引用本文: | 姚娟,高小平.丁苯酞对Wistar大鼠血管性痴呆模型中海马区OX-42的影响[J].湖南师范大学学报(医学版),2016(5). |
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作者姓名: | 姚娟 高小平 |
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作者单位: | 1. 湖南省妇幼保健院特检科,长沙,410008;2. 湖南省人民医院神经内科,长沙,410002 |
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摘 要: | 目的:通过检测Wistar大鼠血管性痴呆模型中小胶质细胞表面补体III型受体OX-42在脑海马组织中的动态变化,探讨丁苯酞在血管性痴呆中的作用。方法:双侧颈总动脉永久结扎术(2-VO)建立Wistar大鼠血管性痴呆模型。设立正常对照组、假手术组、VD模型组、药物干预组。水迷宫试验对大鼠进行学习和记忆成绩测试。应用免疫组织化学法方法检测OX-42的表达。结果:模型组大鼠学习和记忆成绩下降,海马区OX-42的表达较正常对照组、假手术组均明显增加;药物干预组大鼠学习记忆能力明显改善,海马区OX-42的表达下降,与模型组比较差异有统计学意义。结论:慢性脑缺血血管性痴呆大鼠海马区OX-42表达升高;丁苯酞可能通过抑制了血管性痴呆大鼠海马区OX-42的表达,从而改善大鼠的学习记忆能力。
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关 键 词: | 血管性痴呆 OX-42 丁苯酞 Wistar大鼠 |
The effect of NBP on the expression of OX-42 of hippocampus in wistar rats with vascular Dementia |
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Abstract: | Objective To investigate the dynamic variation of OX-42 in hippocampus tissue after vascular dementia(VD) model in Wistat rats, to explore their effects in vascular dementia. To investigate the effects of Butylphthalide(NBP)on OX-42 after VD model in rats, and clarify the possible mechanism of NBP. Methods The vascular dementia modelin Wistar rats were established bypermanentbilateral commoncarotid arteryocclusion method. Wistar rats were divided into 4 groups:normal group, sham-operated group, VD model groupand NBP treatment group. Then the learning and memory capabilities of rats were tested by Morris water maze. Meanwhile, the expression of OX-42were measured with methods of immunohistochemistry. Results Compared with the normal group and sham-operated group, the abilities of learning and memory apparently decresed in VD model group, and the expression of OX-42 significantly increased in VD model group. Besides, NBP treatment groupsignifi-cantlyimproved learning and memory abilities, and compared with the VD model group, the expression of OX-42 significant-lyreduced in NBP treatment group. Conclusion OX-42 expression were significantly increased in hippocampusof chronic cer-ebral ischemia rats. Butylphthalidemight reduced expression of OX-42, and could improve learning and memory abilities of VD rats. |
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Keywords: | vascular dementia OX-42 butylphthalide wistar rat |
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