| 5-烯丙基-7-二氟甲基白杨素诱导肺癌A549细胞生长抑制和凋亡 |
| |
| 引用本文： | 麻小浩,张坚松.5-烯丙基-7-二氟甲基白杨素诱导肺癌A549细胞生长抑制和凋亡[J].湖南师范大学学报(医学版),2009,6(1). |
| |
| 作者姓名： | 麻小浩 张坚松 |
| |
| 作者单位： | 湖南师范大学医学院生理学教研室,湖南长沙,410006 |
| |
| 基金项目： | 湖南省教育厅(06C567);;湖南省科技厅(2007FJ4148) |
| |
| 摘 要： | 目的:研究5-烯丙基-7-二氟甲基白杨素(ADFMChR)诱导人肺癌细胞(A549)细胞生长抑制和凋亡的作用。方法:体外培养人肺癌A549细胞,平皿集落形成法测定细胞锚定依赖性生长能力,软琼脂集落形成法测定细胞非锚定依赖性生长能力,流式细胞术(FCM)分析细胞周期和细胞凋亡率;凝胶电泳观察凋亡细胞的基因DNA梯形条带。结果:平皿集落形成法和软琼脂集落形成法结果显示ADFMChR呈剂量依赖性抑制A549细胞生长,FCM分析发现ADFMChR呈剂量依赖性诱导A549细胞凋亡;ADFMChR(10μg/mL)孵育A549细胞48h后,DNA琼脂糖凝胶电泳呈现典型梯形条带。结论:ADFMChR具有抑制人肺癌A549细胞生长和诱导凋亡作用。
|
| 关 键 词： | 肺癌 白杨素 5-烯丙基-7-二氟甲基白杨素 治疗作用 |
Induction of the Growth Inhibition and Apoptosis in Human Lung Cancer A549 Cell Line by 5-ally-7-gen-difluormethylenochrysin |
| |
| MA Xiao-hao,ZHANG Jian-shong.Induction of the Growth Inhibition and Apoptosis in Human Lung Cancer A549 Cell Line by 5-ally-7-gen-difluormethylenochrysin[J].Journal of Hunan Normal University(Medical Science),2009,6(1). |
| |
| Authors: | MA Xiao-hao ZHANG Jian-shong |
| |
| Institution: | Department of Physiology;Medical College;Hunan Normal University;Changsha 410006;Hunan Province;China |
| |
| Abstract: | Objective To investigate the effect of 5-ally-7-gen-difluormethyleno-chrysin(ADFMChR) on the growth and apoptosis of human lung cancer A549 cells cultured in vitro.Methods A549 cells were treated with various concentrations of ADFMChR.Cell proliferation of A549 cells was evaluated by colony formation in plate and in soft agar test.The apoptosis of A549 cells induced by ADFMChR was determined by DNA fragment assay and flow cytometry with PI staining.Results Data of colony formation in plate and in soft agar ... |
| |
| Keywords: | lung cancer chrysin 5-ally-7-gen-difluormethylenochrysin therapiutic action |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
