千佛菌对老龄小鼠Th_1/Th_2漂移的影响

目的:通过研究千佛菌对D-半乳糖致衰老模型小鼠外周血Th1、Th2型细胞因子(IFN-γI、L-4)水平及I FN-γ/I L-4比值的影响,探讨千佛菌对衰老机体的免疫调节作用。方法:将小鼠随机分为正常对照组、衰老模型组、千佛菌大、小剂量组。除正常组外,其余均腹腔注射D-半乳糖制备衰老模型。千佛菌大、小剂量组分别以100%和50%药液每只每日0.4 mL灌胃,其余等量生理盐水灌胃。测定血清中IFN-γI、L-4含量并计算IFN-γ/IL-4比值,测定脾脏及胸腺指数。结果:模型组小鼠血清IFN-γI、L-4水平I、FN-γ/IL-4比值、脾脏及胸腺指数均明显降低,与正常组比较P0.001;与模型组比较,千佛菌大、小剂量组IFN-γI、L-4水平、脾脏及胸腺指数均明显升高P0.001I,FNγ/IL-4比值恢复;千佛菌大、小剂量组间IFN-γI、L-4比较P0.001。结论:千佛菌对D-半乳糖致衰老模型小鼠Th1、Th2型细胞因子水平及IFN-γ/IL-4比值具有升高作用,并能够显著增加小鼠的脾脏及胸腺指数,对D-半乳糖导致衰老的Th1/Th2免疫失衡具有一定的调节作用,能使之趋向恢复正常。

Effects of QianFoJun on Th1/Th2 Shift in Aged Mice

Objective：To observe the effects of QianFoJun on the level of Th1,Th2 type cytokines（IFN-γ,IL-4） in peripheral blood and the ratio of IFN-γ/IL-4 for aged mice induced by D-galactose and to explore its mechanism of immune regulatory role.Methods：The mice were randomly divided into normal control group,aging model group,QianFoJun large and small dose groups.In addition to the normal group,the rest were injected D-galactose to make model with aging.QianFoJun large and small dose groups were given 100% or 50% drug liquid,orally 0.4mL per day and the remaining groups were given saline gavage.The serum levels of IFN-γ,IL-4 and the spleen and thymus index were determined and the IFN-γ/IL-4 ratio was calculated.Results：The serum levels of IFN-γ,IL-4 levels,IFN-γ/IL-4 ratio,spleen and thymus index in the model group were higher than the normal group;there was significant difference between 2 groups（P〈0.001）.Compared with the model group,the serum levels of IFN-γ,IL-4 levels and spleen and thymus index in the QianFoJun large and low dose group increased;there was significant difference（P〈0.001）;and the IFN-γ/IL-4 ratio was recovered in the QianFoJun large and low dose group.Compared with QianFoJun large and low dose groups in the level of IFN-γ,IL-4,there was significant difference between 2 groups（P〈0.001）.Conclusion：QianFoJun can increase the level of Th1,Th2 type cytokines and the ratio of IFN-γ/IL-4 for aged mice induced by D-galactose and the spleen and thymus index.It had some regulatory role for Th1/Th2 immune imbalance of aged mice induced by D-galactose and made the trend back to normal.