复芪止汗颗粒对小鼠免疫功能影响的实验研究

目的：探讨复芪止汗颗粒对小鼠非特异性免疫和特异性免疫的调节作用。方法：取正常昆明种小鼠40只,雌雄各半,随机分为4组,正常对照组、环磷酰胺模型组、黄芪颗粒组和复芪止汗颗粒组,每组10只。腹腔注射环磷酰胺制备免疫低下小鼠模型。测定胸腺、脾脏质量并计算出小鼠胸腺指数、脾指数;腹腔巨噬细胞吞噬中性红法检测单核-巨噬细胞的吞噬功能;血清溶血素法测定体液免疫功能;脾淋巴细胞增殖实验法测定细胞免疫功能;乳酸脱氢酶释放法测定NK细胞杀伤活性;流式细胞法检测T淋巴细胞亚群的数量。结果：复芪止汗颗粒可显著提高被环磷酰胺抑制的小鼠胸腺指数和脾脏指数;使巨噬细胞吞噬功能明显增加;使血清溶血素水平增高;并能拮抗环磷酰胺对小鼠脾脏T淋巴细胞增殖、杀伤活性、T细胞亚群数量产生的免疫抑制作用。结论：复芪止汗颗粒能增强环磷酰胺所致免疫功能低下小鼠非特异性免疫和特异性免疫功能。

Experimental Study on the Effects of FuQi ZhiHan Granules on Immune Function of Mice

Objective： To approach the accommodation effects of FuQi ZhiHan granules on nonspecific and specific immunity of mice.Method： Forty mice were randomly divided into four groups： control group,model group,groups of HuangQi granules and FuQi ZhiHan granules,ten mice each group.The models were established through injecting cyclophosphamide into abdominal cavity.The qualities of thymus gland and spleen were detected to calculate the indexes of thymus gland and spleen;phagocytosis of the macrophage was determined with neural red assay of peritoneal macrophage phagocytosis;humoral immunity was measured by adopting serum hemolysis;cellular immune function was detected with splenic lymphocyte proliferation;anti-NK cell activity was measured with lactate dehydrogenase release method;numbers of T lymphocyte subgroups were counted with flow cytometric method.Result： FuQi ZhiHan granules can significantly raise thymus gland index and spleen index;improve the phagocytosis of the macrophage;increase serum hemolysin;fight against immunosuppressive action aroused by cyclophosphamide which can promote T lymphocyte proliferation,anti-activity and the quantity of T lymphocyte subgroup.Conclusion： FuQi ZhiHan granules can strengthen nonspecific and specific immunity in mice which show lower immune function caused by cyclophosphamide.