| 高效液相色谱法同时测定复方丹参片6种活性成分含量 |
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| 引用本文： | 李炜,魏玉辉.高效液相色谱法同时测定复方丹参片6种活性成分含量[J].甘肃中医,2012,25(6):23-25. |
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| 作者姓名： | 李炜 魏玉辉 |
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| 作者单位： | 1. 甘肃省人民医院药剂科,甘肃兰州,730000
2. 兰州大学第一医院药剂科 |
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| 摘 要： | 目的:建立同时测定复方丹参片中源于丹参药材的6种活性成分含量的高效液相色谱方法。方法:采用VP-ODS C18柱进行分离(4.6 mm×150 mm,5μm,Shimadzu,Japan);流动相采用1%醋酸(A)和乙腈(B)二元梯度系统;采集波长:263 nm;柱温:40℃;流速:1.0 mL/min。结果:6种活性成分在所建浓度范围内线性关系良好r,2均大于0.999;方法精密度RSD小于4.12%;平均加样回收率均大于98.00%。结论:所建高效液相色谱方法可对复方丹参片中6种活性成分进行含量测定。
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| 关 键 词： | 复方丹参片 高效液相色谱法 含量测定 |
Content Determination of Six Kinds of Active Ingredients in Compound DanShen Tablets with HPLC Simultaneously |
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| LI Wei
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WEI Yu-hui.Content Determination of Six Kinds of Active Ingredients in Compound DanShen Tablets with HPLC Simultaneously[J].Gansu Journal of Traditional Chinese Medicine,2012,25(6):23-25. |
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| Authors: | LI Wei WEI Yu-hui |
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| Institution: | Pharmaceutical Preparation Department of Gansu Provincial Hospital, Lanzhou 730000, China,＂ 2 Pharmaceutical Preparation Department in The First Affiliated Hospital of Lanzhou University |
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| Abstract: | Objective： To establish HPLC methods for content determination of six kinds of active ingredients in compound DanShen tablets simultaneously. Method： Separated with VP-ODS C18 column （4.6 mm×150mm, 5μm, Shimadzu, Japan）; the mobile phase was 1% acetic acid （A）-accetonitrile （B） with gradient elution; detection wavelength： 263nm; column temperature： 40℃ ; flow rate： 1.0 mL/min. Result： Concentrations of six kinds of active ingredients indicated good linear relationships, r2 〉0.999; RSD 〈4.12%; the average recoveries were all higher than 98%. Conclusion： HPLC methods can be used to determine contents of six kinds of active ingredients in compound DanShen tablets. |
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| Keywords: | compound DanShen tablet HPLC content determination |
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