| 细辛脑注射液对槐定碱致痫大鼠Caspase3及热休克蛋白-70表达的影响 |
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| 引用本文： | 甄军丽,王维平.细辛脑注射液对槐定碱致痫大鼠Caspase3及热休克蛋白-70表达的影响[J].中医药研究,2010(3):325-327. |
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| 作者姓名： | 甄军丽 王维平 |
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| 作者单位： | [1]河北医科大学第二医院,050000 [2]河北医科大学第二附属医院,050000 |
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| 基金项目： | 河北省科技攻关基金项目（No.07276101D-21）; 河北省自然科学基金资助项目（No.C2007000852） |
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| 摘 要： | 目的通过对槐定碱致痫大鼠脑内Caspase3及热休克蛋白-70（hsp-70）含量的测定,探讨细辛脑注射液抗癫痫作用的机制。方法随机将42只大鼠分为6组,空白组、模型组、卡马西平阳性对照组及细辛脑低剂量组、中剂量组和高剂量组。通过免疫组化学法和Nissle染色法,研究细辛脑注射液对Caspase3和hsp-70含量的影响。结果研究表明与模型组比较,细辛脑注射液中、高剂量组可显著减轻大鼠癫痫发作（P〈0.05）,可使槐定碱致痫大鼠的脑组织中减少Caspase3释放,降低hsp-70在大鼠脑内的表达。结论细辛脑注射液具有良好的抗癫痫效果,可减轻大鼠癫痫发作时对脑组织的损伤。
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| 关 键 词： | 细辛脑注射液 槐定碱 Caspase3 热休克蛋白-70 |
Effects of Asarone Injection on Caspase 3 and Heat Shock Protein-70 in Sophoridine-induced Epileptic Rats |
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| Zhen Junli,Wang Weiping.Effects of Asarone Injection on Caspase 3 and Heat Shock Protein-70 in Sophoridine-induced Epileptic Rats[J].Research of Traditional Chinese Medicine,2010(3):325-327. |
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| Authors: | Zhen Junli Wang Weiping |
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| Institution: | The Second Hospital,Heibei Medical University(Shijiazhuang 050000) |
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| Abstract: | Objective To explore the anti-epileptic effect and its mechanism of Asarone injection on Caspase 3 and heat shock protein-70（hsp-70）in sophoridine-induced epileptic rats.Methods Forty-two rats were randomly divided into six groups：Blank group,sophoridine-induced epilepsy model group,carbamazepine group,Asarone injection low dosage group,Asarone injection medium dosage group and Asarone injection high dosage group.The content of Caspase 3 and HSP-70 were detected by immunohistochemistry and Nissle staining.Results Compared with model group,the seizure were reduced significantly and the content of Caspase 3 and HSP-70 in brain tissue were decreased in Asarone high dosage group（P 0.05）.Conclusion Asarone injection had anti-epileptic effect,which could play a role in preventing seizure of rats effectively. |
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| Keywords: | Asarone injection sophoridine Caspase 3 heat shock protein-70 |
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