黄连温胆汤加减对代谢综合征大鼠骨骼肌细胞葡萄糖转运子-4的影响

目的：研究黄连温胆汤加减对代谢综合征大鼠骨骼肌细胞 GLU -4的影响及机制。方法：采用膳食诱导建立代谢综合征大鼠模型，将符合成模标准的代谢综合征大鼠随机分为模型组、二甲双胍组、黄连温胆汤低、高剂量组，并给予相应方法干预4周后，检测大鼠骨骼肌细胞 HE 染色，免疫组化法观察骨骼肌细胞 GLU -4的表达。结果：HE 染色结果显示模型组大鼠骨骼肌细胞排列紊乱，部分充血水肿，间隙增宽。药物干预组较模型组明显好转，其中黄连温胆汤高剂量组骨骼肌细胞排列较规整，无明显水肿。免疫组化结果显示各组大鼠骨骼肌细胞均可见 GLU -4的表达，其中黄连温胆汤高剂量组与其他组比较差异具有统计学意义（P ＜0．05）。结论：黄连温胆汤可减轻代谢综合征大鼠骨骼肌损伤，上调骨骼肌细胞 GLU -4的表达。

Effect of Huanglian Wendan Decoction on Skeletal Muscle Cell’s GLU -4 of Metabolic Syndrome Rats

Objective： To explore the effect and mechanism of Huanglian Wendan decoction on skeletal muscle cell ’ s GLU -4.Methods： The male SD rats were established to models of MS by high salt ,high lipid and high glucose diet and the successful model rats were randomly divided into four groups ： the model group,metformin group,Huanglian Wendan decoction low dose group and high dose group .After gavaging the corresponding drug to rats for four weeks ,detect HE staining.Use immunohistochemical method to observe the expression of GLU -4 of skeletal muscle cells.Results： Com-pared with model group,skeletal muscle celld was arranged in order and no obvious edema .The expression of GLU -4 in Huanglian Wendan high dose group had a significant difference （P ＆lt;0.05）.Conclusions： Huanglian Wendan decoction can reduce the metabolic syndrome rats skeletal muscle injury and increase the expression of GLU -4 of skeletal muscle cells.