| 毛脉酸模内生真菌RGT-S4生长曲线及菌丝体化学成分初步研究 |
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| 引用本文： | 王星星,王振月,王博,卫丽曼,郑成琳.毛脉酸模内生真菌RGT-S4生长曲线及菌丝体化学成分初步研究[J].中医药学报,2013(5):17-19. |
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| 作者姓名： | 王星星 王振月 王博 卫丽曼 郑成琳 |
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| 作者单位： | [1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]江苏康缘药业股份有限公司现代中药研究院妇科新药研究所,江苏连云港222001 [3]黑龙江珍宝岛药业药物研究院,黑龙江哈尔滨150000 |
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| 基金项目： | 国家自然科学基金项目（No.30970300）;哈尔滨市科技创新人才研究专项资金项目（优秀学科带头人）（No.2010RFXXS031） |
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| 摘 要： | 目的：研究毛脉酸模（Rumex gmelini Turcz.）内生真菌RGT-S4的生长曲线及菌丝体中的化学成分.方法：运用液体培养法对毛脉酸模内生真菌RGT-S4的生长曲线进行研究,同时运用溶剂萃取、硅胶柱色谱、ODS反相柱色谱、Sephadex LH-20柱色谱、制备液相等方法对其醇提物正丁醇部位进行分离纯化,根据理化性质、NMR数据鉴定化合物的结构.结果：最佳生长天数为7天;同时分离到3个化合物,其结构鉴定为：甘露醇（Ⅰ）、D-果糖（Ⅱ）、L-2-O-甲基-手-肌醇（Ⅲ）.结论：对其生长天数进行研究,以优化其培养条件,同时以上化学成分均为首次从毛脉酸模内生真菌RGT-S4菌丝体中分离得到.
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| 关 键 词： | 毛脉酸模 内生真菌 曲霉属 生长曲线 化学成分 |
Growth Curve and Chemical Constituents of RGT-S4 Mycelium from Rumex Gmelini Turcz |
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| WANG Xing-xingCollege of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin,China ;Institute of Gynecological New Drugs,Modren Institute of Chinese Medicine,Jiangsu Kanion Pharmaceutical Co.Ltd.,Lianyungang,China WANG Zhen-yueCollege of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin,China WANG BoCollege of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin,China ;Zhen Baodao Institute of Pharmaceutical Research,Harbin,China WEI Li-manCollege of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin,China ZHENG Cheng-lin.Growth Curve and Chemical Constituents of RGT-S4 Mycelium from Rumex Gmelini Turcz[J].Acta Chinese Medicine and Pharmacology,2013(5):17-19. |
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| Authors: | WANG Xing-xingCollege of Pharmacy Heilongjiang University of Chinese Medicine Harbin China ;Institute of Gynecological New Drugs Modren Institute of Chinese Medicine Jiangsu Kanion Pharmaceutical CoLtd Lianyungang China WANG Zhen-yueCollege of Pharmacy Heilongjiang University of Chinese Medicine Harbin China WANG BoCollege of Pharmacy Heilongjiang University of Chinese Medicine Harbin China ;Zhen Baodao Institute of Pharmaceutical Research Harbin China WEI Li-manCollege of Pharmacy Heilongjiang University of Chinese Medicine Harbin China ZHENG Cheng-lin |
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| Institution: | WANG Xing-xing(College of Pharmacy, Heilongjiang University of Chinese Medicine ,Harbin 150040, China ;Institute of Gynecological New Drugs, Modren Institute of Chinese Medicine ,Jiangsu Kanion Pharmaceutical Co.Ltd., Lianyungang 222001, China) WANG Zhen-yue(College of Pharmacy, Heilongjiang University of Chinese Medicine ,Harbin 150040, China) WANG Bo(College of Pharmacy, Heilongjiang University of Chinese Medicine ,Harbin 150040, China ;Zhen Baodao Institute of Pharmaceutical Research, Harbin 150000, China) WEI Li-man(College of Pharmacy, Heilongjiang University of Chinese Medicine ,Harbin 150040, China) ZHENG Cheng-lin(College of Pharmacy, Heilongjiang University of Chinese Medicine ,Harbin 150040, China) |
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| Abstract: | Objective：To study the growth curve and chemical constituents of RGT-S4 mycelium from Rumex gmelini Turcz..Method：We studied growth curve by using liquid cultivation,and ethanol extracts by using n-BuOH extraction,solvent extraction,silica gel column chromatography,ODS column chromatography,Sephadex LH-20 and other separation means.The structures were identified according to physical and chemical properties and date of NMR.Results：The best growth days were seven days,and three compounds were identified as Mannitol（Ⅰ）,D-fructose（Ⅱ）,L-2-O-methyl-chiro-inositol（Ⅲ）.Conclusion：Growth days are studied in order to optimizate the culture conditions,and the above compounds are identified from RGT-S4 mycelium of Rumex gmelini Turcz.for the frist time. |
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| Keywords: | Rumex gmelini Turcz Endophytic Fungi Aspergillus Growth curve Chemical constituents |
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