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金钗石斛培养初步研究
引用本文:张艳,范俊安,李泉森,叶代峻.金钗石斛培养初步研究[J].时珍国医国药,2001,12(2):189-190.
作者姓名:张艳  范俊安  李泉森  叶代峻
作者单位:1. 重庆市中药研究院,
2. 重庆市中药研究院,重庆 400065
摘    要:目的筛选金钗石斛的适宜培养基,探索金钗石斛快速繁殖的途径。方法以取材部位、基本培养基、激素比例三因素进行正交试验。结果取材部位是最敏感的因素,其次是激素比例,基本培养基的影响相对较小。结论金钗石斛的适宜培养方案为MS培养基,附加6-BA2~5mg*L-1,NAA0.1~0.5mg*L-1,2000Lx光照10h*d-1,30d左右继代一次。

关 键 词:金钗石斛  组织培养  中药  药物栽培
文章编号:1008-0805(2001)02-0189-01
修稿时间:2000年8月22日

Preliminary Study on the Tissue Cultivation of Dendrobium Nobile Lindl
ZHANG Yan,FAN Jun-an,LI Quan-sen,YE Dai-jun.Preliminary Study on the Tissue Cultivation of Dendrobium Nobile Lindl[J].Lishizhen Medicine and Materia Medica Research,2001,12(2):189-190.
Authors:ZHANG Yan  FAN Jun-an  LI Quan-sen  YE Dai-jun
Abstract:This paper made a priliminary study on the tissue cultivation of Dendrobium nobile Lindl. It was found that the most sensitive factor was the part of the plant used in the tissue cultivation of Dendrobium nobile.The second was the proportion of various planthormones.And the effection of the basic culture medium was relatively weak.The proper cultivating program was:MS culture medium,attached by 6-BA 2~5 mg*L-1 and NAA 0.1~0.5 mg*L-1,(25±3)℃, 2 000 Lx light 10 h*d-1,cultivating a generation about every 30 days.
Keywords:Dendrobium nobile  Tissue cultivation
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