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| 红毛五加叶中常春藤皂苷元的高效液相色谱法测定 | |
| 引用本文： | 钟世红,卫莹芳,古锐,吴宇,段雄. 红毛五加叶中常春藤皂苷元的高效液相色谱法测定[J]. 时珍国医国药, 2010, 21(1) |
| 作者姓名： | 钟世红 卫莹芳 古锐 吴宇 段雄 |
| 作者单位： | 1. 成都医学院·药学院,四川,成都,610083;成都中医药大学,四川,成都,611131 2. 成都中医药大学,四川,成都,611131 3. 成都医学院·药学院,四川,成都,610083 |
| 基金项目： | 国家自然科学基金青年基金项目 |
| 摘 要： | 目的 建立红毛五加叶中常春藤皂苷元的测定方法.方法 采用高效液相色谱法.色谱柱为Welchrom C_(18)色谱柱(4.6 mm×250 mm,5 μm);流动相为甲醇-水-冰醋酸-三乙胺(85:15:0.04:0.02);流速:1.0 ml·min~(-1);柱温:35 ℃;检测波长为210 nm.结果 常春藤皂苷元的线性范围为0.68 ～10.94 μg,平均加样回收率为100.42%,RSD小于2%.结论 该方法准确、专属性强,可为红毛五加叶的综合开发利用提供参考. |
| 关 键 词： | 红毛五加叶 常春藤皂苷元 高效液相色谱 |
HPLC Determination of Hederagenin in Leaves of Acanthopanax giraldii Harms |
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| ZHONG Shi-hong,WEI Ying-fang,GU Rui,WU Yu,DUAN Xiong. HPLC Determination of Hederagenin in Leaves of Acanthopanax giraldii Harms[J]. Lishizhen Medicine and Materia Medica Research, 2010, 21(1) | |
| Authors: | ZHONG Shi-hong WEI Ying-fang GU Rui WU Yu DUAN Xiong |
| Affiliation: | ZHONG Shi-hong1,2,WEI Ying-fang2,GU Rui2*,WU Yu1,DUAN Xiong2(1.Pharmaceutical School,Chengdu Medical College,Chengdu 610083,China,2.Chengdu University of TCM,Chengdu 611131,China) |
| Abstract: | Objective To develop a determination method for the hederagenin in leaves of Acanthopanax Giraldii Harms.Methods Hederagenin was determined by HPLC method. The separation was performed on a welchrom C_(18) column(4.6 mm×250 mm,5 μm)with mobile phase of methanol-water- glacial acetic acid -triethylamine(85:15:0.04:0.02).The flow rate was 1.0 mL ·min~(-1). The column temperature was at 35 ℃. The detection wavelength was 210nm. Results The linear range of hederagenin was 0.68 ～10.94 μg. The average recovery was 100.42%,and the RSD was less than 2%. Conclusion The method is accurate and specific which is useful for the integrative exploitation of the leaves of A. Giraldii Harms. |
| Keywords: | Leaves of Acanthopanax giraldii Harms Hederagenin HPLC |
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