线粒体DNA序列分析鉴定4种蛇粗毒

目的 利用PCR技术鉴定干燥蛇粗毒的来源.方法 从4种蛇粗毒中提取总DNA--其中包括1份已保存了7年的干燥眼镜蛇粗毒,并分别以从蛇粗毒和肌肉组织中提取的总DNA作为模板用1对线粒体16S rRNA基因引物进行扩增.结果 测序结果提交NCBI,并与GenBank中的同源序列进行比对.结论 序列比对和系统发育分析的结果证实,该扩增的即为正确的蛇粗毒来源物种的线粒体16S rRNA基因序列,该技术有可能推广用于对动物粗毒的来源进行准确和直接地鉴定.

Forensic Identification of Snake Crude Venom by mtDNA analysis

Objective Snake venom is a highly valuable industrial raw material. Forensic issues raised by identification of snake products include enforcement of related animal protection regulations, threats to endangered species, and compromise of the integrity of commercial products. Methods In this work, DNA was successfully extracted from four dried snake venoms, among which one sample had been preserved in a pharmaceutical factory for seven years. The mitochondrial 16S gene was amplified and sequenced from the four samples and all sequences were submitted to the National Center for Biotechnology Information (NCBI) and compared against their homologous sequences in the GenBank database. Results Sequence alignment and phylogenetic analysis proved that these samples indeed contained the correct 16S gene from the respective original animals. Conclusion This approach provides a straightforward and accurate method for determining the identity of snake crude venoms.