大孔树脂对葛根总黄酮的吸附及分离纯化研究

以三峡库区丰富的葛根为材料,研究葛根黄酮的分离纯化工艺。方法利用柱层析技术,比较了4种大孔吸附树脂分离纯化葛根总黄酮的效果。结果用AB-8树脂柱进行吸附洗脱,洗脱液为60%乙醇,流速2.4 ml/min,得到的葛根总黄酮(以葛根素计)纯度为53.5%,AB-8树脂的吸附容量为103.2 mg/g。结论建立了AB-8大孔吸附树脂分离纯化葛根总黄酮的适宜条件,发现AB-8大孔吸附树脂在葛根总黄酮的分离纯化中有好的表现。

Research of the Separation and Purification for Flavonoids in Radix Puerarise

Objective Flavonoids were purified from Radix Puerarise of Yichang.Methods Four excellent macroretiaclar resins were selected for separating flavonoid in Radix Puerarise.Results The extract was absorbed by AB-8 macroporous absorption resin and was eluted with 60% ethanol at 2.4 ml/min eluting speed.The purity of total flavonoids was 53.5%.The absorptive capacity of AB-8 resin was 103.2% mg/g.Conclusion A feasible technnology was AB-8 for sublimating the total flavonoids in Radix puerariae.