氧化苦参碱诱导骨肉瘤细胞凋亡的实验研究

目的探讨不同浓度的氧化苦参碱对人骨肉瘤细胞的生长抑制和诱导凋亡作用。方法将不同浓度的氧化苦参碱作用于培养的OS732骨肉瘤细胞,通过MTT比色法、流式细胞仪和DNA凝胶电泳、光学显微镜技术观察检测细胞凋亡,研究分析其对骨肉瘤细胞的生长抑制和诱导凋亡作用。结果浓度为1.77,3.55,5.32mmoL/L的氧化苦参碱对OS732骨肉瘤细胞相对抑制率分别为16.4%,29.0%,47.0%;流式细胞技术显示骨肉瘤细胞的凋亡率分别为11.8%,18.6%,27.7%;DNA电泳见DNA"梯形"图谱;显微镜显示细胞的凋亡形态。结论氧化苦参碱能显著抑制OS732细胞增殖、促进其凋亡。

Experimental Study on the Apoptosis of Osteosarcoma Cells Induced by Oxymatrine

Objective To study the growth-inhibitory effect and the mechanism on apoptosis induced by oxymatrine on OS732 osteosarcoma cells.Methods Osteosarcoma cells were treated with oxymatrine for 3 days.The cyto-toxicity was observed by MTT assay.The apoptotic rates were shown by flow cytometry(FCM).Electron microscopy and DNA gel electrophoresis were applied to demonstrate of the presence of apoptosis.Results When osteosarcoma cells were treated with oxymatrine(1.77,3.55 and 5.32 mmol/L),the cytotoxicity indexes were 16.4%,29.0% and 47.0% respectively,the apoptostic rates were 11.8%,8.6%,27.7%.The obvious apotosis morphological changes in osteosarcoma cells were shown by electron microscope.Conclusion Oxymatrine is able to inhibit the proliferation and induce the apoptosis of OS732 cells significantly.It is a potential and valuable proposal for clinical treatment of osteosarcoma.