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| 不同来源黄芪多糖提取物对淋巴细胞增殖的影响及其化学组成分析 | |
| 引用本文： | 谢兴亮,盛艳梅,韩丽,杨明. 不同来源黄芪多糖提取物对淋巴细胞增殖的影响及其化学组成分析[J]. 时珍国医国药, 2011, 22(11). DOI: 10.3969/i.issn.1008-0805.2011.11.059 |
| 作者姓名： | 谢兴亮 盛艳梅 韩丽 杨明 |
| 作者单位： | 1. 成都医学院药学院,四川成都,610083 2. 成都中医药大学药学院,四川成都,611137 3. 江西中医学院现代中药制剂教育部重点实验室,江西南昌,330004 |
| 基金项目： | 国家重大新药创制专项，成都医学院自然科学基金 |
| 摘 要： | 目的 考察不同来源黄芪多糖提取物对体外脾淋巴细胞增殖的影响,明确其活性差异,对比分析其化学组成.方法 MTT法分别检测黄芪多糖提取物对正常脾淋巴细胞、ConA活化脾淋巴细胞增殖的影响；硫酸苯酚法结合重量法分别测定样品中总糖、多糖、醇不溶组分含量.结果 各批样品对正常脾淋巴细胞均有显著的促增殖作用(P＜0.01或P＜0.05),除SSK外,各样品在低浓度时促增殖作用较强,促增殖率为39.4％ ～ 186.4％;XZX、XHS、SLH1对ConA诱导的脾淋巴细胞增殖具有显著抑制作用(P＜0.01),且在4 mg·ml-时抑制作用最强,抑制率为52.9％～104.0％,作用大小为XZX＞XHS＞SLH1,其余各批样品无明显抑制作用；各批样品醇不溶组分含量为18.74％～60.67％,多糖含量为7.22％～44.74％,大小顺序为XZX＞ XHS＞ SLH1＞ SLH2＞ SSK.结论 不同来源黄芪多糖提取物的质量差异十分显著. |
| 关 键 词： | 黄芪多糖 淋巴细胞增殖 化学组成 不同来源 |
Effect of Astragalus Polysaccharides Extract from Different Sources on Lymphocyte Proliferation and its Chemical Composition Analysis |
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| XIE Xing-liang,SHENG Yan-mei,HAN Li,YANG Ming. Effect of Astragalus Polysaccharides Extract from Different Sources on Lymphocyte Proliferation and its Chemical Composition Analysis[J]. Lishizhen Medicine and Materia Medica Research, 2011, 22(11). DOI: 10.3969/i.issn.1008-0805.2011.11.059 | |
| Authors: | XIE Xing-liang SHENG Yan-mei HAN Li YANG Ming |
| Affiliation: | XIE Xing-liang1,SHENG Yan-mei1,HAN Li2,YANG Ming3 (1.Pharmacological College,Chengdu Medical University,Chengdu 610083,China,2.Pharmacological College,Chengdu University of Traditional Chinese Medicine,Chengdu 611173,3.Key Laboratory of Modern TCM Preparation,Ministry of Education,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China) |
| Abstract: | Objective To investigate the effect of Astragalus polysaccharides extract from different sources on lymphocyte proliferation,illuminate the difference of immunocompetence,and compare their chemical composition. Methods MTT method was used to detect the effect of lymphocyte proliferation.Gravimetric analysis was used to determine the content of composition in dissoluble in alcohol,and phenol-sulferic acid method determine the contents of total sugar,polysaccharide in different samples by phenol-sulferic acid... |
| Keywords: | Astragalus polysaccharide Lymphocyte proliferation Chemical composition |
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