| 家蝇幼虫分泌物对小鼠腹腔巨噬细胞功能的影响 |
| |
| 引用本文： | 国果,吴建伟,付萍,秦荣贵,张勇,谢佩文.家蝇幼虫分泌物对小鼠腹腔巨噬细胞功能的影响[J].时珍国医国药,2011,22(10). |
| |
| 作者姓名： | 国果 吴建伟 付萍 秦荣贵 张勇 谢佩文 |
| |
| 作者单位： | 1. 贵阳医学院·基础医学院,贵州贵阳5 50004
2. 贵阳医学院·药学院,贵州贵阳 550004 |
| |
| 基金项目： | 国家自然科学基金(No.30960343); 贵州省教育厅自然科学基金(No.F2008-6); 贵阳医学院基金项目(No.C2007-2) |
| |
| 摘 要： | 目的 研究家蝇幼虫分泌物对小鼠腹腔巨噬细胞吞噬功能、乳酸脱氢酶(LDH)活性及一氧化氮(N0)含量的影响.方法 以甲醇为洗脱液,用固相萃取法初步分离家蝇幼虫分泌物,液体培养法检测各萃取组分抗菌活性,比色法检测萃取组分对巨噬细胞吞噬中性红能力及LDH活性的影响；Griess法测定NO含量.结果 分泌物经甲醇萃取分离后,30％,40％萃取组分显示较强抗大肠埃希菌活性;经该两个梯度萃取组分作用后,巨噬细胞的吞噬率分别为(1.48±0.12)％,(1.26±0.08)％; LDH活性单位分别为(450.32±1.15)及(306.57±2.06)；诱生NO的浓度分别为(28.5±3.4) μmol/L,(25.6±2.8) μmol/L,与对照组相比均有明显的统计学意义(P＜0.05).结论 家蝇幼虫分泌物经初步纯化后,对小鼠腹腔巨噬细胞吞噬功能、LDH活性及NO的诱生均有显著的增强作用.
|
| 关 键 词： | 家蝇幼虫 分泌物 小鼠 巨噬细胞功能 |
Effect of Housefly Larvae Secretion on Macrophage Function in Mice |
| |
| GUO Guo,WU Jian-wei,FU Ping,QIN Rong-gui,ZHANG Yong,XIE Pei-wen.Effect of Housefly Larvae Secretion on Macrophage Function in Mice[J].Lishizhen Medicine and Materia Medica Research,2011,22(10). |
| |
| Authors: | GUO Guo WU Jian-wei FU Ping QIN Rong-gui ZHANG Yong XIE Pei-wen |
| |
| Institution: | GUO Guo1,WU Jian-wei1*,FU Ping1,QIN Rong-gui2,ZHANG Yong1,XIE Pei-wen1(1.Basic Medical College,Guiyang Medical University,Guizhou 550004,China,2.Pharmacological College,China) |
| |
| Abstract: | ObjectiveTo study the effect of housefly larvae secretion on phagocytic function,nitric oxide content and the activity of lactate dehydrogenase. MethodsHousefly larvaes secretion isolation was performed by solid phase extraction.The antibacterial effect of secretion was studied in broth method.The percentage of phagocytosis of neutral red(NR) by mouse peritoneal macrophages and the activity of lactate dehydrogenase were detected by colorimetric assay.Nitric oxide(NO) production was examined by Griess reacti... |
| |
| Keywords: | Housefly larvae Secretion Mice Macrophage function |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
